Sentences with phrase «chromatin immunoprecipitation»

Hanner and Rusche used chromatin immunoprecipitation assays to show that this chimeric protein did not make it to the HMRa or HMRα loci or to other repressed genes.

The authors next used chromatin immunoprecipitation (ChIP) assays to directly show that the K14A mutation in histone H3 allowed Sgo1p to bind when the TSM was mutated.

In the study, the team performed a genome - wide chromatin immunoprecipitation analysis for genes that were the target of BMAL1, a core clock component that binds to many other clock genes, regulating their transcription.

Rao and Bredesen next used genome - wide chromatin immunoprecipitation sequencing (ChIP - seq) to determine where in the genome the two ApoE isoforms bound.

To evaluate histone modification (another epigenetic event), the authors employed chromatin immunoprecipitation with high - throughput sequencing (CHiP - Seq) to capture DNA sequences bound by H3 histones marking active (K4me1, K4me3, K27ac, K36me3) or repressed (K27me3, K9me3) chromatin.

(D) Cells transfected with either Keap1 or control siRNA were subjected to chromatin immunoprecipitation after treatment with either 10 µM HPP - 1014, 10 µM CoPP, or 1 µM HPP - 4382 for 6 hours.

(A) NHLF Cells were treated with 0.1 µM CDDO - Me or 1 µM HPP - 4382 for 6 hours after which they were crosslinked with 1 % formaldehyde in media, washed, and collected to be processed for chromatin immunoprecipitation as described in Materials and Methods.

Chromatin immunoprecipitation verified that HPP - 4382 treatment of NHLF cells reciprocally coordinated a decrease in binding of Bach1 and an increase of Nrf2 binding to the HMOX1 E2 enhancer.

One current method commonly used today is called chromatin immunoprecipitation, combined with next - generation gene sequencing (ChIP - seq).

At ABclonal, we have nearly 100 existing chromatin immunoprecipitation (ChIP)- validated antibodies with a higher specificity than most commercial antibodies, which are used for a wide range of epigenetics studies, from those investigating developmental processes to those researching various diseases.

For example, the 2015 Nature scientific report, Important biological information uncovered in previously unaligned reads from chromatin immunoprecipitation experiments (ChIP - Seq) discusses how various computational tools can find new information that can assist in the construction of gene regulatory grids.

Comprehensive study of the chromatin events during hematopoiesis (or any other development process) has been hampered by the low sensitivity of current Chromatin immunoprecipitation (ChIP) protocols.

To evaluate whether loss of ABL kinases affected TAZ activity, we performed chromatin immunoprecipitation (ChIP) analysis using primers for TAZ targets identified by ChIP sequencing analysis (42).

This unique technology was launched in December 2017, and integrates automated chromatin immunoprecipitation and next generation sequencing library preparation within the same protocol, eliminating the traditional tedious steps, purifications, and inefficiencies that can result from standard ChIP - seq protocols.

Furthermore, genome - wide studies, combining chromatin immunoprecipitation (ChIP) and array hybridization (ChIP - on - chip), have revealed that both active and silenced genes are directly bound in ES cells by one or more of the core pluripotency factors Oct4, Sox2 and Nanog [17], [19], [63].

STAT6 co-immunoprecipitated with the transcriptional co-activator, p300, and chromatin immunoprecipitation assays demonstrated that these proteins bind a consensus STAT6 binding site located within the COX - 2 promoter.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

Chromatin immunoprecipitation sequencing analysis revealed enrichment of H3K27me3 at specific loci in KDM6A - null cells, including PRC2 / EZH2 and their downstream targets.

We further probed the ability of HPP - 4382 to modulate transcription factor binding to the HMOX1 promoter via chromatin immunoprecipitation.

(C) Cells transfected with either Nrf2 or control siRNA were subjected to chromatin immunoprecipitation after treatment with 1 µM HPP - 4382 for 6 hours.

We confirmed that these compounds modulate Bach1 directly using chromatin immunoprecipitation and reporter assays.

Services include global and gene - specific DNA methylation analysis, and chromatin immunoprecipitation (ChIP) followed by library preparation in a high - throughput format (HT ChIP - Seq).

All chromatin immunoprecipitations were quantified using quantitative PCR.

Chromatin immunoprecipitation was used to monitor Nrf2 and Bach1 occupancy at the HMOX1 E2 ARE.

Chromatin immunoprecipitation assay.

Chromatin immunoprecipitation (ChIP) assay was performed using EZ ChIP kit (Millipore) according to the instruction of the manufacturer.

Chromatin immunoprecipitation (ChIP) is a technique to investigate protein - DNA interactions at a specific genomic - site.

Chromatin immunoprecipitation was performed as previously described (Lee et al., 2006).

Chromatin immunoprecipitation assay of the MELK promoter in MDA - MB - 468 cells (right).