Sentences with phrase «culture medium containing»

Stable transfectants were selected in culture medium containing G418 (1mg / ml).

Following cultivation of Arabidopsis seedlings on a culture medium containing a high concentration of cesium carbonate, Cesium Green was applied to the seedlings, and the resulting green fluorescence observed was used to confirm the presence of cesium within the plants» cells.

But if the culture medium contains an anti-inflammatory molecule, this response is blocked.»

Not exact matches

Samples collected from surfaces and from the air are cultured on plates containing a growth medium, one specific for bacteria and another for fungi.

Although electrolyte leakage is still undesired, its danger is minimized by the use of either the normal saline solution pumped into the body in most IV treatments or a cell - culture medium that contains amino acids, sugars, and vitamins in addition to sodium ions and thus mimics the fluid that surrounds human cells.

Traditionally, hMSCs have been cultured on two - dimensional cell culture platforms using serum - containing medium.

By culturing cells in a fed - batch system with StemSpan ™ medium containing cytokines and UM171, expanded cells were found to successfully engraft and repopulate immunocompromised mice, with no disadvantage when compared to unmanipulated cells.

To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red.

For induction and culture of mouse iPS cells, HFF cells were passaged using trypsin and cultured in medium containing DMEM, 10 % FBS (Hyclone), 1 % NEAA (Invitrogen), 2 mM L - glutamine (Invitrogen), 100 U / ml penicillin and 100 µg / ml streptomycin.

SH - SY5Y cells were cultured in Dulbecco's modified Eagle's medium nutrient mixture F - 12 (DMEM / F12; Sigma), supplemented with 10 % FBS (Sigma), 100 nM l - glutamine (Sigma), 100 U / ml penicillin (Sigma), 10 µg / ml streptomycin (Sigma), in a humidified atmosphere containing 5 % CO2 at 37 °C.

A single - cell suspension (50 μl) containing 1500 cells was mixed with Matrigel (1:1) and plated on top of the Matrigel base onto wells of a 96 - well plate; 50 μl of complete medium was added, and the cells were cultured for 14 days.

Cells were plated in 12 - well plates at 0.8 × 105 cells / well (A427) or 1.8 × 105 cells / well (H2122) in RPMI medium containing 10 % FBS and cultured overnight.

Cells were plated in six - well plates at 2.5 × 105 (A427) or 4 × 105 (H2122) cells / well in RPMI 1640 medium containing 10 % FBS and cultured for 24 h. Culture medium was collected, and PGE2 levels were determined using a PGE2 EIA kit (Cayman Chemicals).

Cells were cultured at 37 °C in an atmosphere of 5 % CO2 in Rosewell Park Memorial Institute (RPMI) 1640 medium (Mediatech, Herndon, VA, USA) containing 10 % fetal bovine serum (FBS)(Gemini Bio-Products, Woodland, CA, USA), 100 U / ml penicillin / streptomycin and 2 mM glutamine (Invitrogen, Carlsbad, CA, USA).

These inserts are added to wells containing complete culture medium with 10 % FBS as chemoattractant in 24 - well fluorescence opaque plates allowing only monitoring of fluorescence from the bottom.

The skin biopsies were washed in Ca / Mg - free Dulbecco's Phosphate Buffered Saline (PBS, Invitrogen, Carlsbad, CA, http://www.invitrogen.com) and minced into approximately 12 smaller pieces before being seeded onto gelatin - coated 6 - well cell culture plates (Corning Enterprises, Corning, NY, http://www.corning.com) containing mouse embryonic fibroblast (MEF) media consisting of Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 % fetal bovine serum (FBS, Invitrogen) and 100 IU / ml penicillin - streptomycin (Invitrogen), and cultured at 37 °C in a 5 % CO2 incubator.

The harvested cells are transferred to a Petri dish containing a specialized nutrient broth, known as a culture medium.

The pearls contain natural prebiotics that poses as the ideal medium for the culture and growth of the good bacterium in them

Encapsulated with its own prebiotic culture medium — Most probiotic supplements contain the probiotic only.

The supernatant, the medium in which the culture is grown, contains a multitude of beneficial byproducts of the growth process, including vitamins, enzymes, antioxidants, and immune stimulators.

Most veterinary practices use «dermatophyte test medium» as their culture medium, because it contains a color indicator that turns the medium red when ringworm starts to grow on it.

The sample (possibly) containing parasitic eggs is isolated and grown in a culture medium.