Sentences with phrase «cumulus cell study»

Not exact matches

In collaboration with Anna Pyle, Howard Hughes Medical Institute investigator and professor in the Department of Molecular, Cellular and Developmental Biology at Yale, Patrizio and his team studied samples from 20 cumulus cells in 15 patients younger than age 35 and in those age 40 and older.

As outlined below, we used a microfluidic quantitative PCR (qPCR) system to elucidate the gene expression profiles of individual human oocytes and small numbers of cumulus cells using a combination of a large number of samples and targets [12], and then extended our studies via the use of parthenogenesis, in conjunction with gene expression profiling, as a functional assay of cytoplasmic maturation of oocytes.

Six ovarian factors (BDNF, IGF - I, estradiol, GDNF, leptin and FGF2) were further studied based upon the expression of their receptors in oocytes and cumulus cells described above, as well as the availability of growth factor concentration information from previous animal model studies [1], [20], [21], [22], [23].

Expression of the mRNA encode for Glial cell line - Derived Neurotrophic Factor (GDNF) was detected in cumulus cells as shown previously [22] as well as oocytes in our study.

Oocytes, cumulus cells and frozen embryos were collected from Stanford Fertility and Reproductive Medicine Center after approval by the Stanford Institutional Review Board (IRB); all samples were obtained with written informed consent from all participants involved in the study.