Grant 1557: High - Resolution
Cytogenetic Analysis of Histiocytic Malignancies and Development of a Targeted Assay to Screen for Expression Level Changes Dr. Matthew Breen, PhD Project Goal: The goal of this project is to narrow down the search for genes playing a key role in Histiocytic Malignancies and thus move a step closer to developing targeted therapies for canine patients diagnosed with this devastating cancer.
The AKC CHF - funded NC State CVM research projects are: Grant 1557: High - Resolution
Cytogenetic Analysis of Histiocytic Malignancies and Development of a Targeted Assay
Not exact matches
The primary goal
of this facility is to offer conventional and molecular
cytogenetic services, including species identification, karyotyping,
analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping
A large variety
of methods is used including genetic, molecular and
cytogenetic techniques, fluorescence in situ hybridisation and DNA «combing», as well as high throughput sequencing approaches such as DNA - seq for mutation landscape
analyses, RNA - seq for transcriptome
analyses and ChIP - seq mapping
of chromatin - associated proteins and their genome - wide modulation in response to DNA damages.
Cytogenetic and immuno - FISH
analysis of the 4q subtelomeric region, which is associated with facioscapulohumeral muscular dystrophy.
Cytogenetic analysis now extends beyond the simple description
of the chromosomal status
of a genome and allows the study
of fundamental biological questions, such as the nature
of inherited syndromes, the genomic changes that are involved in tumorigenesis and the three - dimensional organization
of the human genome.
Allele - specific PCR, molecular
cytogenetic studies, microsatellite PCR, Affymetrix single nucleotide polymorphism array
analyses, and colony assays were undertaken on subgroups
of patients.
Expansion
of ES cells generated outside
of MDACC and
cytogenetic analysis / Service Request form.
The techniques
of karyotyping such as culturing, harvesting, staining and chromosomal
analysis need patience, accuracy and particular skills and I had proved to be an excellent and highly skilled
cytogenetic technologist
Sound knowledge
of Cytogenetics principles, FISH procedures, and DNA / RNA extraction and
analyses