Sentences with phrase «cytometry in»

At these time points, mixed chimerism was monitored by flow cytometry in venous blood, spleen and bone marrow.

(B) The expression of MHC antigens by the chimeric hematopoietic cells was determined by flow cytometry in the Rag2 − / − γc − / − mice.

Expression of Venus and SSEA - 1 were quantitated by flow cytometry in two independent clonal lines and compared to both control and parental cells.

Quantitative Flow Cytometry in Cell Therapy Ines Mende, PhD, Hitachi Chemical Advanced Therapeutic Solutions, LLC

Mass cytometry, or CyTOF, is a variation of flow cytometry in which antibodies are labeled with heavy metal ion tags rather than fluorochromes.

A new joint International Dairy Federation (IDF) / International Organization for Standardization (ISO) Standard (ISO 19344 IDF 232) has just been released to provide a method for the quantification of lactic acid bacteria by flow cytometry in fermented products, starter cultures, and probiotics used in dairy products...

Not exact matches

There will be several rounds of recruitment, and «specifically at this time, we are accepting applications for both junior / senior postdoctoral researchers and recent graduates wishing to enroll in our Ph.D. program as well as personnel with extensive expertise in cell culture and one expert in flow cytometry to fill in the position of manager of the flow - cytometry platform.»

CST has about 225 employees, and specializes in making antibodies to be used in research applications such as immunohistochemistry and flow cytometry.

Also covered will be techniques for analyzing exosomes, microvesicles, and apoptotic bodies in unprocessed samples, how imaging flow cytometry can be used to evaluate or reevaluate EV isolation techniques, and the advantages and disadvantages of using this method.

Flow cytometry analysis using mouse blood showed that MSCs were released from bone marrow to the blood in cinnamtannin B -1-administered mice.

Imaging flow cytometry (IFC) has emerged as a useful and efficient tool for studying the signaling pathways in immunophenotypically defined subpopulations of immune cells.

Dr. Maguire is a senior cytometry specialist in the department of Flow and Image Cytometry at the Roswell Park Cancer Institute in Buffalo, cytometry specialist in the department of Flow and Image Cytometry at the Roswell Park Cancer Institute in Buffalo, Cytometry at the Roswell Park Cancer Institute in Buffalo, New York.

He has worked in the biotech industry as a research scientist for over 11 years with a focus on emerging technologies including gene targeting in mice, molecular analysis of transgenes using GFP variants at the single cell level, and developing flow cytometry reagent kits to speed up assay development time for researchers.

Secondary antibodies play an important role in many research applications, including immunofluorescence, immunohistochemistry, Western blot, ELISA, flow cytometry, and more.

Using flow cytometry, in which fluorescent probes are used to detect IgA - coated bacterial species, the researchers discovered that IgA - coated E. coli were abundant in fecal samples from patients with both Crohn's disease and spondyloarthritis.

Employing the advanced technology of the Institute's Cytometry, Imaging and Histology centers, the researchers examined the relationship between megakaryocytes and hematopoietic stem cells in mouse bone marrow.

The two plots in the Nature paper show flow cytometry data - a counting up of cells - after exposure to other cells in bone marrow taken from younger and older mice.

Analytical flow cytometry analysis was performed on whole blood after lysis of erythrocytes and fixing in 2 % PFA.

Together the stable disruption of CXCR4 as determined by both the surveyor nuclease assay and flow cytometry suggests that CXCR4 disruption did not negatively impact cell viability or growth in humanized NSG mice over a two - month period.

Flow cytometry and sorting data were analyzed with FlowJo software (Treestar) or Cellquest (BD Biosciences), in the case of primary cells.

Flow cytometry of SVCs isolated from two perigondadal adipose tissue depot illustrates that tissue from macrophage - deficient mice (b) contains 34 % the number of F4 / 80 + cells found in adipose tissue from control mice (a).

IDMIT will contribute 1) To the development and validation of assays based on flow cytometry and mass cytometry for the evaluation of immune responses in humans and animal models; these tools will be particularly relevant for the identification of signatures of vaccine efficacy; 2) To the animal model platform, in particularly by providing access to NHP models and to new technologies for in vivo imaging infections and host responses; 3) To networking activities, in particular by organising a workshop on in vivo imaging.

The immune responses will be evaluated in the different species by measuring influenza antigen - specific IgG / IgA and IgM, neutralising antibodies and antibodies inhibiting hemagglutination and antigen specific lymphocyte responses and cytokine secretion profiles by advanced flow cytometry.

IDMIT is an infrastructure for preclinical research in infectious diseases and immunology which is certified ISO9001 and which includes 1) A large animal facility with capacity to host NHP in BSL2 and BSL3 containment, 2) State - of - the - art laboratories for cell biology, immunology, molecular biology, flow cytometry and mass cytometry (CyTof), cell - sorting and confocal microscopy in BSL3 containment; 3) A biological resources centre with high storage capacity; 4) Highly innovative technologies for in vivo imaging of large animals in BSL2 and BSL3 containment, including a two - photon microscope, a PET - CT facility, and several optic based technologies (fibered endo - microscopy, near infra - red imaging).

Dr. Ruxandra Sîrbulescu analyzes flow cytometry data on immune cells used in a wound healing study.

ab9484 staining GAPDH in A549 lung cancer cells by Flow Cytometry.

The team has access to state - of - the - art laboratories and animal facilities with BSL2 and BSL3 containment, flow cytometry, cell sorting, histopathology and in vivo imaging (IVIS).

Moreover, the allospecific apoptotic activity of the putative DN T cells was abolished in two separate experiments using in vitro treatment with anti-Thy 1.2 antibody plus complement, which removed > 97 % of the Thy 1 + cells, as determined by flow cytometry.

ab85137 staining S100 in a human melanoma cell line by Flow Cytometry.

Current Protocols in Cytometry, 2 - 21.

Works well in flow cytometry and immunohistochemistry.

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

Flow Cytometry: This four day program is ideal for those who are looking for an in - depth, hands - on introduction to Flow Cytometry.

Quick and reliable assessment of chronological life span in yeast cell populations by flow cytometry.

The flow cytometry facility at the Wellcome Sanger Institute is a dedicated scientific service offering investigators with state of art flow cytometry instrumentation together with assistance in running samples, data analysis and experimental design.

Someone who knows a thing or two about the importance of data collection is Michael Andreeff, M.D., Ph.D., professor of Leukemia, who was a pioneer in the develop of flow cytometry, a method for counting and sorting cells that is commonly used in today's clinical trials.

(d) Histogram of membrane - bound Tie2 expression in HUVEC and BP compared to isotype control measured by flow cytometry.

All of our team members have many years of experience in different aspects of flow cytometry.

Surface expression of Tie2 in BP and HUVEC as well as isolated mBP was analysed by flow cytometry.

In accordance with the flow cytometry data, expression of AcGFP1 in gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cellIn accordance with the flow cytometry data, expression of AcGFP1 in gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cellin gesicle - treated cells was knocked out in the majority of cells, despite AcGFP1 being expressed in nearly all control cellin the majority of cells, despite AcGFP1 being expressed in nearly all control cellin nearly all control cells.

In total, 106 splenocytes were cultured without peptide or with 0.2 μM LCMV gp33 peptide, NP396 peptide for 5 h. IFN - γ positivity by flow cytometry was used to identify LCMV - specific cells (13).

We are using CyTOF mass cytometry and single cell RNA - Sequencing to identify new monocyte subsets that may be important in disease.

These finding were exactly in accordance with Gillet et al., 1996 [24], who stated that, Cyclin D1 staining did not appear to correlate with an increased S - phase fraction as judged by flow cytometry.

In harmony to the present suggestion, the scoring and image cytometry evaluation using immunohistochemistry of Cyclin D1 protein marker stated that the variations of protein expression in tissue sections could yield prognostic information or could be useful in determining subtle effects of curative or prevention therapies [31In harmony to the present suggestion, the scoring and image cytometry evaluation using immunohistochemistry of Cyclin D1 protein marker stated that the variations of protein expression in tissue sections could yield prognostic information or could be useful in determining subtle effects of curative or prevention therapies [31in tissue sections could yield prognostic information or could be useful in determining subtle effects of curative or prevention therapies [31in determining subtle effects of curative or prevention therapies [31].

The percentage of CSCs in the cycling hypoxia selected subpopulation was analyzed based on the CD44, CD24, ESA, and E-cadherin expression by three - color flow cytometry.

Research Focus: I am highly trained in producing and purifying monoclonal antibodies from Hybridoma cells, performing ELISA assays, preparing primary cells from organs and analyzing cell populations using FlowJo Cytometry data analysis software, isolating genomic DNA from animal tissues, maintaining and screening numerous transgenic and knock out mice lines by PCR and FACS.

Flow cytometry and high - content analysis experimental data will be presented to demonstrate performance in receptor occupancy, antibody internalization, and effector function assays using antibodies against CD20, CD71, and HER2 in breast cancer and leukemia models.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.

No significant correlation was observed between Cyclin D1 staining and S - phase % obtained by flow cytometry as illustrated in Table 5.

By supporting the development of innovative, cutting - edge applications in the areas of super-resolution microscopy, flow cytometry, Raman spectroscopy and metrology, the Cobolt lasers can contribute to advancing the understanding and defeating of severe diseases, to improvements in quality of life and a better environment.