Sentences with phrase «ddx3 nuclear staining»

In our experiments, both HNF4α and ASGPR1 are well - expressed in enhanced hiPS - HEP cells from all three hiPS cell lines (Figure 2), as shown by homogeneous nuclear staining for HNF4α and homogeneous membrane staining for ASGPR1.

DDX3 was strongly immunolocalized in the nucleus and less intensively in the cytosol of squamous cells in normal skin, but the nuclear staining was lost or dramatically decreased in cutaneous SCC.

Some cases with strong nuclear staining, the cytoplasm was also stained.

Panels with blue color show DAPI nuclear staining.

To further confirm this observation, the intensity of nuclear staining and the percentage of nuclear positive cells obtained from immunohistochemistry analysis of 17 normal epidermis and 34 SCC specimens were analyzed statistically.

As regard to cyclin Dl expression, the present result showed that it was mostly nuclear staining with variable range of stain intensity between different sections in the individual groups.

The intensity of DDX3 nuclear staining and the percentage of DDX3 nuclear staining positive cells in normal skin and cutaneous SCC tissues are summarized.

The intensity of nuclear staining was divided into four levels: negative, slight, moderate, and strong.

The remaining 6 cases (20 %) did not show any considerable staining for Cyclin D1 in the nucleus and were classed as negative nuclear stain.

Immunofluorescent nuclear staining of STAT6 in H2122 cells.

For secondary antibodies, the sections were incubated with Alexa Fluor 546 - conjugated donkey anti-goat IgG antibody (Molecular Probes, Eugene, OR), Alexa Fluor 488 - conjugated donkey anti-rabbit IgG antibody (Molecular Probes) or Alexa Fluor 488 - conjugated donkey anti-mouse IgG antibody (Molecular Probes), Alexa Fluor 546 - conjugated goat anti-mouse IgG2b, or Alexa Fluor 488 - conjugated goat anti-mouse IgM, and then were counterstained with 1 µg / ml Hoechst 33258 for nuclear staining.

Values of calcein retention (efflux function) were normalized to Hoechst nuclear staining (viable cell density).

Cells were stained with anti-human IgG (green) and DAPI nuclear stain (blue).

(B) DAPI nuclear staining.

XMRV - SO orange staining, CEP8 - SA aqua staining and DAPI nuclear staining were visualized, respectively, with filters of orange, aqua and DAPI (Abbott Molecular, Inc.).

Not exact matches

By July, with a reelection campaign looming and Albany's dirt staining his image, Cuomo resorted to what an aide termed «the nuclear bomb»: his Moreland Commission on state corruption.

If nuclear counterstaining is desired, four - color antibody staining is possible using BV421, BV480, Alexa Fluor 488, and Rhodamine Red - X.

After obtaining cells from 31 species of nine rodent families, Gallardo's group used a stain to estimate the amount of nuclear DNA in each cell.

C, immunohistochemical analysis for KLF4 expression was conducted for primary breast tumors and brain metastatic tumors, and the numbers of nuclear or cytosolic staining were assessed under microscope.

(A) Rosettes (arrowheads) and pseudo outer nuclear layers (arrow) are detected in explants triple stained for nuclei (blue; DAPI), cone (green; Calbindin), and rod photoreceptors (red; XAP2).

Blue is DAPI, staining nuclear DNA.

Morphologic examination of the Hoechst - stained cell preparations at 16 hours after treatment with 20 nmol / L rapamycin showed morphologic evidence of apoptosis, such as nuclear condensation and fragmentation in ALCL cells (A, bottom right).

This result was agreed with several previous studies who stated that, Cyclin D1 showed nuclear and cytoplasmic staining and had an extensive range of intensities between different sections [22, 23].

Specifically, the percentage of nuclear immunoreactive cells was graded into five categories: 0 % to 10 % cells stained, 11 % to 25 % cells stained, 26 % to 50 % cells stained, 51 % to 75 % cells stained, and 76 % to 100 % cells stained.

Immunohistochemistry - Paraffin: Emx1 Antibody - Staining of human kidney shows moderate nuclear positivity in cells in a subset of renal tubules.

Confocal images of P150 dystrophic retina transplanted with hNPCctx — GDNF and double stained with antibodies against human nuclear antigen (red) and either (A) recoverin, a photoreceptor and cone bipolar cell marker (green), or (B) protein kinase Cα (PKCα), a bipolar cell marker (green).

(C) Retinal section from the same eye used in panel A stained with proliferating cell nuclear antigen (PCNA) revealing occasional dividing cells in the RPE - L layer and inner retina at P150 (arrows).

A nuclear reference staining (DAPI) is visualized in blue.

(C) Confocal image of P150 dystrophic inner retina transplanted with hNPCctx — GDNF and double stained with antibodies against nestin, a neural stem and progenitor cell marker (green) and human nuclear antigen (red).

Basic annotation parameters include an evaluation of i) staining intensity (negative, weak, moderate or strong), ii) fraction of stained cells (rare, < 25 %, 25 - 75 % or > 75 %) and iii) subcellular localization (nuclear and / or cytoplasmic / membranous).

Stained for DNA with DAPI (Blue), the nuclear envelope with LaminB1 (Green) and the centrosome marker γ - tubulin (Red).

Blue is nuclear DNA stain.

(A) Extensive preservation of the nuclear photoreceptor layers in the dorsal retina of the dystrophic RCS rat 13 weeks following transplantation of iPS - RPE cells (DAPI stained nuclei).

Every 50th section was stained with hematoxylin and eosin for nuclear boundaries and counterstained with Luxol fast blue for myelin boundaries.

Joining the discussion again are Alan Wilson of Tripwire Interactive (Killing Floor, Red Orchestra), Kedhrin Gonzalez of Illfonic (Nexuiz), Alex Austin of Cryptic Sea (A New Zero), Michiel Beenen of Interwave (Nuclear Dawn), Oscar Jilsén of Coffee Stain Studios (Sanctum), and Mladen Bošnjak of Misfit Village (SickBrick).