(B) Southern
blot analysis of EcoRI - digested genomic
DNA from wt, Xpd † XPCS / wt, and Xpd † XP / wt recombinant embryonic stem cell clones hybridised with the 3 ′ probe depicted in (A).
DNA preparation, western
blot, immunofluorescence, immunohistochemistry, isobolographic
analysis, mRNA - seq library preparation and sequencing
analysis, ChIP - seq library preparation and sequencing
analysis, the TUNEL assay, murine model and micro-positron emission tomography and computed tomography imaging are described in the Online Supplementary Methods.