We also work closely with colleagues within the department and university and have built photon counting, laser induced fluorescence systems for capillary electrophoresis with Professor Steiner,
DNA Electrophoresis imaging systems with Professor Mendis, graphene functionalization and Chromone Laser Dye Synthesis with Professor Li.
It miniaturises a technique called
DNA electrophoresis.
Not exact matches
Common tests are performed on large, highly sophisticated automated analysers, while more specialised tests are performed using technology such as absorption spectroscopy,
electrophoresis, HPLC, GC - MS, and tandem MS, immunoassay, and
DNA analysis.
They come to the teaching lab at FHCRC and spend a day extracting
DNA, micropipetting, and learning about gel
electrophoresis.
The NLISA platform uses a process call gel
electrophoresis to screen synthesized, single strands of
DNA «reagents» that change their shape in the presence of a specific biomarker.
Vijayvargia's team of master's degree students recently designed and built a
DNA gel
electrophoresis unit with power pack and ultraviolet illuminator that would cost as much as $ 2000 commercially; their version cost about $ 35.
The ultimate goal is to put not just
electrophoresis, but the preparation of
DNA and the analysis of the
DNA patterns all onto a single device controlled by a built - in computer.
Gel
electrophoresis is a process through which different proteins or
DNA fragments are separated so that they can be identified and studied.
We hope that this result will accelerate the automation and miniaturization of gel
electrophoresis, which in turn can make medical diagnoses and
DNA sequencing cheaper, faster, and more reliable,» says Assoc. Prof. Nathaniel Robinson, leader of the research group, on their «work as a logical extension of previous studies on conducting polymer electrodes in electrokinetic systems.»
After a few days, we isolate the
DNA from the cells, amplify it with the polymerase chain reaction, and use
electrophoresis to show that the CD32 gene has been cut into pieces.
The resulting
DNA fragments are analyzed by agarose gel
electrophoresis.
The improved size resolution of the
DNA chip allows students to identify their genotype, something impossible with agarose gel
electrophoresis.
Large - scale and effective studies of biological molecules with a different structural basis such as proteins, nucleic acids, carbohydrates, lipids and numerous metabolites (including e.g. fatty acids, amino acids, purines, pyrimidines or steroids) is currently highly attractive because of the availability of modern instruments and tools for both separation and analysis (2 - D chromatographs, capillary
electrophoresis systems, microfluidics, mass spectrometers, powerful NMR spectrometers,
DNA / RNA arrays and others).
Have basic equipment and skills to collect samples, extract and amplify
DNA by PCR, and confirm by gel
electrophoresis.
The primary component is polyacrylamide, known for its use in soft contact lenses and as the
electrophoresis gel that separates
DNA fragments in biology labs; the second component is alginate, a seaweed extract that is frequently used to thicken food.
He also pioneered the development of microfabricated capillary
electrophoresis devices and microfabricated integrated sample preparation and detection methods for lab - on - a-chip analysis that are being applied to
DNA sequencing, diagnostics, forensics, pathogen detection and space exploration.
Using Surveyor nuclease followed by gel
electrophoresis, these mismatches are indicated by the presence of
DNA cleavage products.
This activity introduces
DNA analysis through
DNA digestion with restriction enzymes and agarose gel
electrophoresis.
Primer specificity was assessed by melting temperature analysis, gel
electrophoresis and direct sequencing (Iowa State
DNA Facility, Ames, IA).
Other uses of
electrophoresis include
DNA testing, where electricity is used to separate the parts of a
DNA sample by making them move across a gel, which enables them to be compared with other samples and identified.
The AKC - CHF grant will cover the cost of the renal biopsy / autopsy evaluation (if this is performed), urinalysis, serum chemistry, urine protein gel
electrophoresis, and
DNA isolation (approximately $ 850 value).
We use morphometrics, allozyme
electrophoresis, mitochondrial
DNA (mtDNA) restriction - site analysis, and analysis of hypervariable minisatellite
DNA to measure variability within and distances among island fox populations.
First, she will use the clinical method of gel
electrophoresis (commonly used to visualize
DNA) to conduct a «hormone race» between cells composed of different ratios of estrogen and testosterone.
Tags for this Online Resume: research and development,
DNA, qPCR, MSD, Biacore, octet, Capillary
electrophoresis
Tags for this Online Resume: Analytical chemist, Biochemist, RNA or
DNA, Mass spectrometry, microRNA, sequencing, MALDI, Assay Development, capillary
electrophoresis
• Adept at teaching chromatography and gel
electrophoresis • Able to develop and implement chemistry curriculum and lessons aimed at assisting students in learning complex concepts such as organic and chemical chemistry • Strong knowledge of sequence specific recognition principals of
DNA binding properties and its implementation in a classroom environment • Complete understanding of preparing interactive course materials including chemistry pilot projects to introduce new chemistry concepts
His product experiences include clinical chemistry, special chemistry, histology, immunohistochemistry,
electrophoresis, immunoassay, HPLC, microarray, MA and
DNA separation and purification.
Responsible and highly motivated professional with solid and diverse experience as Molecular Biologist with excellence in isolating and purifying
DNA, bacterial plating, polyacrylamide gel
electrophoresis, agarose gel
electrophoresis.
Tags for this Online Resume: ELISA,,
Electrophoresis (SDS - PAGE, Western Blotting), Chromatography (TLC, CE)-RRB--RRB-, Spectrophotometery (Bradford, A280, BCA)-RRB-, Cell culture, cell proliferation, RNA,
DNA, Q PCR, Virology, Viral Safety Testing (in - vitro, retrovirus, reverse transcriptase assays,
Electrophoresis (SDS - PAGE, Western blot), Chromatography (TLC, CE, HPLC), Spectrophotometry (Bradford, A280, BCA)
Tags for this Online Resume: Ecl (Control Lang), Purchasing, Software, ARIA, Injections, Intelligent Call Management, Research, Transcription, Genotyping, Western blot, viral vectors,
DNA cloning, RNA extraction, RNA syntehesis, confocal microscopy, cell culture, transfection, transduction, western blot, PCR, RT - qPCR, Next Generation
DNA sequencing, ion torrent, gromacs, ICM, Schrodinger Tools, NCBI software, DIVA, FlowJaw, Flow Cytometry, Cell Sorting, Aria II, Aria III, XCalibur, TaqMan,
electrophoresis, Sanger
DNA sequencing, FCS Express, cell patterning, ELISA, immunoassay, antibody, HPLC, his - tag, flag - tag, flash - tag, chromatography, stem cells,
DNA extraction, CRISPR, cas9, plasmid, lentivirus, CAR - technology, MLV, Chimeric Antigen Receptors, Jurkat, U87, HeLa cells
Professional Duties & Responsibilities Biomedical and biotechnology engineer with background in design of biomaterials, biosensors, drug delivery devices, microfrabrication, and tissue engineering Working knowledge of direct cell writing and rapid prototyping Experience fabricating nanocomposite hydrogel scaffolds Proficient in material analysis, mechanical, biochemical, and morphological testing of synthetic and biological materials Extensive experience in bio-imaging processes and procedures Specialized in mammalian, microbial, and viral cell culture Working knowledge of lab techniques and instruments including
electrophoresis, chromatography, microscopy, spectroscopy, PCR, Flow cytometery, protein assay,
DNA isolation techniques, polymer synthesis and characterization, and synthetic fiber production Developed strong knowledge of FDA, GLP, GMP, GCP, and GDP regulatory requirements Created biocompatible photocurable hydrogels for cell immobilization Formulated cell friendly prepolymer formulation Performed surface modification of nano - particle fillers to enhance their biocompatibility Evaluated cell and biomaterial interaction, cell growth, and proliferation Designed bench - top experiments and protocols to simulate in vivo situations Designed hydrogel based microfluidic prototypes for cell entrapment and cell culture utilizing computer - aided robotic dispenser Determined various mechanical, morphological, and transport properties of photocured hydrogels using Instron, FTIR, EDX, X-ray diffraction, DSC, TGA, and DMA Assessed biocompatibility of hydrogels and physiology of entrapped cells Evaluated intracellular and extracellular reactions of entrapped cells on spatial and temporal scales using optical, confocal, fluorescence, atomic force, and scanning electron microscopies Designed various biochemical assays Developed thermosensitive PET membranes for transdermal drug delivery application using Gamma radiation induced graft co-polymerization of N - isopropyl acylamide and Acrylic acid Characterized grafted co-polymer using various polymer characterization techniques Manipulated lower critical solution temperature of grafted thermosensitive co-polymer Loaded antibiotic on grafted co-polymer and determined drug release profile with temperature Determined biomechanical and biochemical properties of biological gels isolated from marine organisms Analyzed morphological and mechanical properties of metal coated yarns using SEM and Instron Performed analytical work on pharmaceutical formulations using gas and high performance liquid chromatography Performed market research and analysis for medical textile company Developed and implement comprehensive marketing and sales campaign