Sentences with phrase «dna microarray»

Manager, Target Now / Horizon — Molecular Profiling Institute — Phoenix, AZ 2005 to 2007 Oversaw clinical intake for all patients for Target Now program comprised of esoteric Immunohistochemistries and DNA Microarray for patients with advanced cancer.

DNA Microarray (LS.1 j; LS.13 f; BIO.5 j) This is a BioInteractive Click n» Learn Activity that teaches students the process of working with large data sets created by microarrays.

In recent years, many labs have dedicated enormous resources to identifying new molecular cancer markers through «unbiased» direct profiling of human cancers using DNA microarray approaches.

DNA microarray chips are used to detect missing segments of chromosomes (deletions) or additional segments (duplications) that may be too small to be seen by karyotype.

We assessed the ability of a commercial DNA microarray to characterize bovine Shiga toxin - producing Escherichia coli (STEC) isolates and evaluated the results using in silico hybridization of the microarray probes within whole genome sequencing scaffolds.

DS carried out cell culture experiments and JLE performed DNA microarray assay and corresponding data analysis.

This will be achieved by exposing the coral to seawater with high calcium concentrations to induce an alteration in gene expression profiles, identified using a DNA microarray analysis.

Xu applied the DNA microarray technique to screen more than 100,000 genes in the human genome to find the exact gene regulation pathway.

To understand why only some motor neurons are vulnerable to ALS, the researchers used DNA microarray profiling to compare the activity of tens of thousands of genes in neurons that resist ALS (oculomotor neurons / eye movement and Onuf's nuclei / continence) with neurons affected by ALS (lumbar 5 spinal neurons / leg movement).

He had spent half of his graduate years working with one small DNA microarray data set, but Millennium Pharmaceuticals in nearby Cambridge was «printing microarrays to beat the band; they had hundreds.»

That nightmare scenario came true for Brooks and a host of other researchers last spring when a popular DNA microarray design proved to be faulty.

Other labs have used DNA microarray analysis to characterize gene expression changes in this model.

Whether you are studying the expression of thousands of genes simultaneously using DNA microarrays, or the interaction of multiple molecules to understand intercellular signalling, the skills that will help you understand your findings are statistics, computational techniques, and modelling — so all you really need is a mathematician.

DNA microarrays consist of thousands of spots contained on a single 1 x 3 inch glass slide, each spot representing a different gene.

We currently produce DNA microarrays representing the yeast, mouse, and human genomes (the yeast microarray has over 6000 yeast genes, the mouse microarray has over 15,000 mouse genes, and the two human microarrays have 1700 genes and over 19,000 genes).

By measuring gene expression in the transgenic mice using DNA microarrays, researchers discovered 50 biomarkers of gene expression that each indicated declining kidney function.

To study the variations, Chess and his colleagues turned to DNA microarrays.

DNA microarrays, also called DNA chips, have stoked excitement in the research community because they can test the activity of many genes at once.

Gene expression patterns in the hippocampus samples were examined using DNA microarrays that measured the expression levels of over 30,000 genes (transcripts) per sample.

As mRNA isolation is simple and since DNA microarrays are a proven genomics reagent for monitoring differential gene expression, a genome - wide expression analysis may be the fastest and most efficient method for identifying additional candidate effectors of colony queen number.

The Gladstone team had profiled changes in gene expression using DNA microarrays, which provides an unbiased method for identifying key biological pathways.

To reveal the process as to how this occurs, the researchers created the first full - mouse genomic platform of DNA microarrays.

«I think these diagnostic polymer foils are very interesting alternatives to standard DNA microarrays, especially when it comes to point of care diagnostics», says Associate Professor Aman Russom, group leader of the Clinical Microfluidics group in the Nanobiotechnology laboratory at KTH.

The students are employing that technology — which includes CRISPR (clustered regularly interspaced short palindromic repeats) systems, DNA microarrays, real - time polymerase chain reactions, RNA sequencing and western blotting — to study acute myeloid leukemia (AML), which affects both children and adults and comes with a high mortality rate for older patients.

With the development of DNA microarrays, the gene chips that allow scientists to read the output of thousands of genes at once, along with robotics and computers, scientists tear through hefty analyses with previously unheard - of swiftness.

We chose the three most selective primers for use in printing DNA microarrays.

We have designed DOP - PCR primers specifically for the amplification of large insert clones for use in the construction of DNA microarrays.

In DNA Microarrays (Methods Express Series)(Schena M, ed), Scion Publishing, Ltd., Bloxham.

But when the researchers used DNA microarrays to survey the content of genes in each genome, they found significant differences among the strains.

Not exact matches

Therefore, before publication, large data sets (including microarray data, protein or DNA sequences, atomic coordinates or electron microscopy maps for molecular and macromolecular structures, and climate data) must be deposited in an approved database and an accession number or a specific access address must be included in the published paper.

The Emory team used high density microarrays to probe more than 900,000 sites across the human genome to detect structural variation, including deletions or duplications of DNA.

Large - scale methods of probing samples, such as DNA sequencing, microarrays, and automated gene - function studies, are filling new databases to the brim.

ONE OF THE FIRST THINGS MIKOVITS DID was to employ a microarray — a small tray seeded with DNA from nearly every known virus — to flag viral DNA in human white blood cells.

And he had some preliminary evidence: When he added DNA from malignant prostate tissue cells to a microarray, a novel retrovirus emerged.

Because of uncertainties about the orientation of DNA fragments, workers inadvertently jumbled some of the microarrays.

Here we show that hybridization capture on microarrays can successfully recover more than a megabase of target regions from Neandertal DNA even in the presence of ~ 99.8 % microbial DNA.

As a research associate in the pathology labs of Yale School of Medicine, Mrs. Canosa has worked extensively with microarray expression profiling, genotyping, and DNA sequencing.

One of the techniques she uses is SNP microarrays, which enable researchers to screen a genetic sample for many SNPs at once on specially designed DNA «chips».

In essence, microarrays are fingernail - sized chips embedded with snippets of known DNA.

Using microarrays that enabled them to examine 100,000 sites on the genome, they looked at the cells» nuclear DNA, mitochondrial DNA, and methylation patterns (which give clues to whether a gene has been turned on or off).

Band DNA was labeled with Cy5 and hybridized to microarrays with Cy3 - labeled whole genome DNA.

Services provided by EMBL Heidelberg include the Core Facilities which provide internal users, collaborators and visitors with access to excellent facilities for high - throughput analysis including microarrays, protein production, mass spectrometry, DNA sequencing, advanced light and electron microscopes and chemical genomics.

We work very closely with the Sequencing and Microarray Facility (SMF) and the DNA / RNA samples extracted from us can be submitted directly from our core to SMF across the hallway.

Using a specialized DNA array containing thousands of Arabidopsis genes (an EST microarray), the researchers then analyzed gene expression in their mutant and quickly found that MPK4 somehow controls the expression of anti-microbial genes of the SAR response.

We used custom microarrays to capture and Illumina sequence 6 Mb of targeted intronic DNA, and combined this dataset with published protein coding sequences derived from transcriptome sequencing [28]--[29].

Biotin - l... Keywords: Arylations, Cross couplings, DNA replication, Diagnostic, Gene expression, Microarray Analysis, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing

Geographical location of the populations sampled for the transcriptome (red circles) and DNA hybridization on microarrays datasets (black circles).

We obtained polymorphism and divergence data from six individuals for each of the rabbit subspecies using Illumina sequencing of genomic regions enriched by DNA hybridization on microarrays [60].

We also describe a method for the bulk testing of the hybridization characteristics of chromosome - specific clones spotted on microarrays by use of DNA amplified from flow - sorted chromosomes.

The microarray - based services also are provided for RNA expression profiling and DNA genotyping.