A particular focus was germline editing, which refers to changing
the DNA of fertilized eggs or embryos.
Not exact matches
The
DNA in the nucleus
of the
fertilized egg contains all the instructions needed to make all the different proteins and all the different sorts
of structures in all the different sorts
of cells in the body.
The method, called somatic cell nuclear transfer (SCNT), replaces the
DNA in an
egg cell's nucleus with the genetic material from the nucleus
of a skin cell, then tricks the
egg cell to start dividing as if it had been
fertilized with sperm.
That hunk
of DNA was supposed to be a template that the
fertilized egg could use to repair the breach made by Cas9.
As part
of these ongoing research activities, the
DNA of Dravet patients without SCN1A mutations was analyzed by trio exome sequencing, which searches across the active parts
of the genome for de novo mutations that have arisen in these patients (de novo mutations are
DNA copying errors that occur in the parents» gametes or in the
fertilized egg or embryo, resulting in the afflicted family member being the first person in their family to have this genetic condition).
«Maybe at the one - cell or two - cell stage,» Eggan and his colleagues reasoned, «there's still some
of that stuff in there...» And if they picked the right moment
of cell division, when these powerful reprogramming factors were still floating around in the periphery
of the cell, they might be able to use drugs to temporarily freeze the cell in the middle
of division, stick in the needle
of a micromanipulator to suck out the embryonic
DNA, squirt in
DNA from an adult animal, and then kick - start the process
of reprogramming — hours, perhaps even days after an
egg had been
fertilized.
Now it appeared that natural selection was operating within each
fertilized human
egg, as each parent's
DNA competed for control
of the developing offspring, each with a different evolutionary goal.
Although the researchers had first removed the
DNA of both the
egg and the
fertilizing sperm, the
egg was still primed for cell division and development, increasing the chances
of success, the team says.
Although the U.K's new coalition government plans to disband the agency later this year, HFEA today announced a scientific review into a potential new fertility treatment that involves swapping
DNA from one woman's
fertilized egg into that
of another.
A complementary
DNA encoding the human low density lipoprotein (LDL) receptor under control
of the mouse metallothionein - I promoter was injected into
fertilized mouse
eggs, and a strain
of mice expressing high levels
of LDL receptors was established.
They found some changes to the
DNA in the space samples, but not enough to affect the ability
of the sperm to
fertilize eggs or produce baby mice.
In the case
of zebrafish, less than 20 percent
of fish produced by injecting
DNA into
fertilized eggs will transmit the foreign gene to the next generation.
In regular old human reproduction, sperm
fertilizes an
egg, combining two sets
of DNA into one.
The research, conducted in Benin City in southern Nigeria, suggests toxic metals and other contaminants in e-waste are reducing levels
of key hormones in men in developing countries and could contribute to reduced sperm levels, and cause damage to the sperm's
DNA, stability and ability to
fertilize eggs.