Sentences with phrase «dna recombination»

He completed his postdoctoral studies in gene targeting and DNA recombination at Massachusetts Institute of Technology (MIT).

Unique DNA structures, such as four - way and three - way junctions related to DNA recombination and other processes, are being structurally analysed.

These genes are assembled by DNA recombination and code for proteins that are a crucial part of antibodies, produced in antibody - secreting lymphocytes or B - cells.

E. Revenkova, M. Eijpe, C. Heyting, C. A. Hodges, P. A. Hunt, B. Liebe, H. Scherthan and R. Jessberger SMC1b is Required for Meiotic Chromosome Dynamics, Sister Chromatid Cohesion, and DNA Recombination.

Site - specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1.

The Nobel Assembly announced Professor Sir Martin as one of three winners for «a series of ground - breaking discoveries concerning embryonic stem cells and DNA recombination in mammals.»

The study of heteroplasmy is proving to be useful in the study of mutation patterns, the role of selection and the mitochondrial DNA recombination in mammals.

«We found that when PP4 was missing, chromosomes failed to assemble correctly and DNA recombination, an important step for genetic diversity, did not occur,» said Aya Sato - Carlton a researcher involved in the study from iCeMS.

Not exact matches

But since this DNA is inherited only from the maternal line, it does not go through the normal genetic process of division and recombination.

The Hin recombinase binds to specific sites on DNA and mediates a recombination event that results in DNA inversion.

They trick cells into treating the added DNA as its own, greatly increasing the chance of homologous recombination.

Not least the fact that gene targeting using homologous recombination or any other method is not perfect — the added DNA is sometimes inserted into the wrong place in the genome, and the process can trigger other kinds of mutations too.

Recombination - based approaches in vivo are especially uncommon, because for most DNA constructs recombination has a very lowRecombination - based approaches in vivo are especially uncommon, because for most DNA constructs recombination has a very lowrecombination has a very low probability.

Mutation - based models argue that sexual reproduction reduces mutations, which suggests the recombination of DNA during...

Immunoglobulin K genes are constructed during lymphocyte differentiation by the joining of two DNA elements, VK and JK, to form both a VKJK coding unit and a reciprocal recombination product.

DNA elements are asymmetrically joined during the site - specific recombination of kappa immunoglobulin genes

This recombination of DNA rings into the long chromosomes of today's animal species would have allowed proteins to evolve much more swiftly than simple mutational changes would allow, argues Edward Trifonov of the Weizmann Institute of Science in Rehovot, Israel.

«If the main DNA repair pathway, BRCA - mediated homologous recombination, becomes defective, cancer cells adapt and still proliferate.»

But BRCA is just one of the many genes that encode important proteins in the DNA repair pathway known as homologous recombination,» says the study's lead author, Arielle Heeke, MD, a clinical fellow at Georgetown Lombardi Comprehensive Cancer Center.

Because the chromosomes of each parent undergo genetic recombination during meiosis, each gamete, and thus each zygote, will have a unique genetic blueprint encoded in its DNA.

RecA protein is essential in eubacteria for homologous recombination and promotes the homologous pairing and strand exchange of DNA molecules in vitro.

But by combining the drug with a PGAM1 inhibitor to impair both homologous recombination and PARP - dependent DNA repair, the researchers were able to significantly suppress tumor growth.

Cells lacking the BRCA1 ubiquitin ligase activity were found to be sensitive to certain DNA damaging agents that need homologous recombination in for repair.

This was because the cells were unable to activate a pathway called homologous recombination that repairs this type of DNA damage.

The new research has found that this attachment of ubiquitin by BRCA1, its «ubiquitin ligase activity», is needed for a specific type of DNA repair that is «error - free», known as homologous recombination.

Previously, cancer researchers surmised that since chromosomal ends get swapped in ALT, mutation of genes that restrain DNA exchange, a process scientists call recombination, might actually cause the condition (or, to extend the ping - pong metaphor, make cells lose paddle control).

In molecular biology, recombination generally refers to the molecular process by which genetic variation found associated at two different places in a continuous piece of DNA becomes disassociated (shuffled).

«What the PNA does is clamp to the DNA close to the mutation, triggering DNA repair and recombination pathways in cells,» Egan explained.

However, in Plasmodium falciparum, this approach can take up to a year because it relies on homologous recombination, a type of genetic swapping that cells use to repair broken DNA strands.

Analysis of retroviral recombinants formed after a single round of replication revealed that (i) the nonselected markers changed more frequently than expected from the rate of recombination of selected markers; (ii) the transfer of the initially synthesized minus strand strong stop DNA was either intramolecular or intermolecular; (iii) the transfer of the first synthesized plus strand strong stop DNA was always intramolecular; and (iv) there was a strong correlation between the type of transfer of the minus strand strong stop DNA and the number of template switches observed.

These data suggest that retroviral recombination is ordered and occurs during the synthesis of both minus and plus strand DNA.

Median progression - free survival with niraparib compared to placebo was 21.0 vs 5.5 months in the germline BRCA mutation group (hazard ratio [HR] 0.27, 95 % confidence interval [CI] 0.173 to 0.410, p < 0.0001), 9.3 months vs 3.9 months in the non-germline BRCA mutation group (HR 0.45, 95 % CI 0.338 to 0.607, p < 0.0001), and 12.9 vs 3.8 months in a subgroup of the non-mutation cohort who had homologous recombination DNA repair deficiencies (HRD)(HR 0.38, 95 % CI 0.243 to 0.586, p < 0.0001).

Using «homologous recombination,» CRISPR can also be used to insert new DNA sequences or even whole genes into the genome in a precise way.

Cohesins are protein complexes that bind two straps of DNA and are implicated in its repair, replication and recombination, as well as in its chromosomal stability, transcription regulation, stem - cell pluripotency, and cell differentiation.

To assemble the largest pieces of DNA, they inserted them into yeast cells and exploited a natural process called «homologous recombination,» which is used by yeast to repair damaged DNA.

Scientists have suspected that, like cells in the immune system, olfactory neurons diversify through recombination — that is, each cell randomly shuffles its DNA so it carries a slightly different version of the same gene.

Through a process called homologous recombination, cells automatically replace the bad DNA with the patch.

One thing known to boost homologous recombination is a break in the DNA.

Much of this DNA is no longer capable of moving, but is likely «auditioning» perhaps as a regulator of gene function or in homologous recombination, which is a type of genetic recombination where the basic structural units of DNA, nucleotide sequences, are exchanged between two DNA molecules to repair breaks in the DNA strands.

from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA

Ordinarily, a long continuous stretch of DNA would be broken up by the reshuffling of maternal and paternal chromosome segments that occurs in each new generation (known as homologous recombination).

In that year, Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV infection, gene targeting vectors with relatively short (1 kb) homology arms underwent homologous recombination at a frequency three orders of magnitude higher than identical targeting vectors delivered through transfection of naked DNA.

While the biology behind this AAV effect remains unclear, it is likely not a coincidence that the AAV genome is composed of single stranded DNA, which is particularly well suited for strand invasion, a key step in the homologous recombination pathway.

Approximately 50 % of epithelial ovarian cancers (EOC) exhibit defective DNA repair via homologous recombination (HR) due to genetic and epigenetic alterations of HR pathway genes.

As alluded to above, the Cre recombinase catalyzes the site specific recombination event between two loxP sites, which can be located either on the same or on separate pieces of DNA.

The idea here is that the donor DNA acts as a DNA repair template that will be inserted at the DSB during homologous recombination (HR).

Both genes function in the homologous recombination (HR) pathway of the DNA double - strand break (DSB) repair...

In this edition, we're going to take a look at a very interesting tool that can be used for creating (excuse the pun) specific, targeted DNA modifications in transgenic animals, embryonic stem cells, and / or tissue - specific cell types: Cre - lox recombination.

BRCA1 / 2 proteins function in homologous recombination (HR)- mediated DNA repair and cooperate with Fanconi anemia (FA) proteins to maintain genomic integrity through replication fork stabilization.

Activated IGF - 1R inhibits hyperglycemia - induced DNA damage and promotes DNA repair by homologous recombination.