Hailing from academia and biotechnology, we aimed to discover
differences in gene expression levels between tumors and healthy cells.
The only clue indicating that
differences in gene expression level are not the reason for the observed correlation between the recombination rate and the size of introns is that we do not see a comparable pattern when examining the size of exons, the number of exons per gene, and the overall size of proteins.
Not exact matches
The new study, published this month by Molecular Autism
in a special issue on sex
differences in autism, further shows a stronger correlation between the
expression level of RORA and that of
genes regulated by RORA
in males.
If, for instance, a researcher finds that dozens of
genes in a microarray are differentially expressed on a high - fat purified ingredient diet compared to a low - fat GB diet, it is tempting to conclude that
gene expression was altered due to the
differences in fat
levels between the diets.
Others were unanticipated, including significant
differences in expression levels among
genes involved
in fundamental cellular processes such as ribosomal biogenesis, transfer RNA processing, and Notch - signaling — part of a complex system of communication that governs basic cellular activities and coordinates cell actions.
To quantitatively determine
differences between the
expression levels of these
genes in hNSCs and hESCs, we utilized real time RT - PCR.
The ability of these populations to interconvert
in vitro combined with their subtle
differences in gene expression lead us to ask if there was any functional significance to this low
level of PrEn
gene expression.
Using 5 % false discovery rate
in ANOVA calculation
in any of the 3 comparisons (CGR8 vs. E14TG2a vs. R1), there are 137
genes (0.9 % of the analyzed transcripts) that show a 2-fold
difference or higher
in expression levels among the three lines; 34 of these
genes are > 2-fold higher or lower expressed
in E14TG2a, 5
in CGR8 and 11
in R1 cells.
We considered a minimum ten fold
difference in the
expression levels of
genes as a relevant criterion for classifying them as high and low
expression genes.