Sentences with phrase «e-flo electroporation»

Using Pulse Electric Field Processing (PEF) the patent protected E-FLO Electroporation System provides significant product improvement in chip crunch, taste and texture as well as reduction in acrylamide formation and oil content.

Using electroporation — in Corn's words, «we run an electric current and make holes in the cells» — he'll inject the complex and replacement parts through the cells» protective membrane.

Compared to conventional microinjection, the electroporation method was easy to carry out, fast and scalable.

This new electroporation delivered the genome - editing molecular for generating mutant animals.

DeWitt places a plastic tray about the size of a microwave dinner, with 16 thimble - sized wells filled with the culture, under the hood of the electroporation device.

«New innovative method for delivering genes into cells: Modification of cell physiological function with novel parallelized electroporation of mammalian cells by using electrostatic manipulation in a water - in - oil droplet.»

The first authors, Assistant Professor Hirofumi Kurita and Professor Akio Mizuno, said, «W / O droplet electroporation is operated using a DC power supply, which obviates the need for an expensive pulse generator.

However, most commercial electroporation - based transfection methods require the use of specialized pulse generators to produce short electrical pulses at high voltage.

There have been improvements in W / O - droplet electroporation electrodes for use in disposable 96 - well plates for concurrent performance (Fig. 2).

In addition, the size of the droplet is quite small compared with that in conventional bulk electroporation, resulting in the use of fewer materials in high - throughput analysis.»

Associate Professor Rika Numano explained, «The W / O droplet electroporation technique has several advantages over conventional transfection techniques: small cell number required, as low as 1000 cells; small amount of DNA required, which makes it applicable for various cell types including neural cells; and changeable genome DNA.

They then used a method known as electroporation, in which cells are briefly exposed to an electrical field that makes their membranes more permeable, to quickly deliver these RNPs to the interior of the cells.

The article, «Retrobulbar Inflammation, Adipogenesis and Acute Orbital Congestion in a Preclinical Female Mouse Model of Graves» Orbitopathy Induced by Thyrotropin Receptor Plasmid - in Vivo Electroporation,» will be published in the September issue of Endocrinology.

H. Efficient CRISPR / Cas9 - Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease.

The amenability of the chick embryo to manipulation and imaging, combined with the powerful technique of in vivo electroporation and the strong similarities of muscle formation in birds and mammals provide a unique paradigm to characterize this process in amniotes.

Expansion of embryonic and adult neural stem cells by in utero electroporation or viral stereotaxic injection.

For both BayGenomics parental ES cell line E14Tg2a.4 (also used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple electroporations and germline transmission are available.

Produced by electroporation of MT - 2 cells with pxxHIV - 1Lai proviral DNA.

To generate your own edited clonal hiPS cell lines, try one of our complete systems containing a Cas9 - sgRNA RNP complex delivery system (choose electroporation - based or gesicle - based delivery) and the Cellartis iPSC Single - Cell Cloning DEF - CS Culture Media Kit:

INO - 3112 is injected into a muscle followed by electroporation, a technique that uses controlled, millisecond electrical pulses that make it easier for the DNA plasmids to enter muscle cells.

This group cultured human CD34 + cells in StemSpan ™ medium and cytokines as a pre-stimulation step ahead of electroporation, before xenotransplantation into mice or additional in vitro culture.

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Tissues targeted (as reported in animals): Primarily blood, immune, and stem cells removed from the body; localized electroporation to skin

Ex vivo cortical electroporation (E15 mouse embryos) coupled with organotypic culture for 5 days results in radial migration of EGFP + pyramidal neurons to the cortical plate (CP) and the projection of their axon in the intermediate zone (IZ).

Primarily blood, immune, and stem cells removed from the body; localized electroporation to skin

A cortical layer 2/3 neuron at 21 days in vitro labeled by ex utero electroporation with fluorescent marker for spine visualization.

Cell culture steps were used by both groups to let cells recover after thawing and / or electroporation, and additionally to generate erythroid progenitor cells from edited CD34 + cells [1].

Synaptic reconstruction of all excitatory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation (Unpublished, Dan Iascone)

Synaptic reconstruction of all excitatory and inhibitory inputs made onto a single cortical pyramidal neuron labeled with sparse in utero electroporation.

Though speaking of which, I should go ask if electroporation counts for the «electrical shocking of an organism» badge...

HV cells constitutively expressing the LacZ gene were generated by electroporation with a vector containing a CAG driven β - Geo cDNA followed by selection in G418 (150 µg / ml) for 2 wk.

When tested in retinal electroporation studies, DsRed reporter expression was induced in ~ 76 % of GFP + cells, and 100 % of DsRed + cells also expressed GFP.

Important reports from the Weiner lab include the first DNA vaccine studied for HIV as well as for cancer immune therapy of cutaneous T cell lymphoma, the early development of DNA encoded genetic adjuvants, including IL - 12, advances in gene optimization, and advances in electroporation technologies resulting in improved gene delivery.

Electroporation of cultured cells was carried out using the Neon Transfection System (Invitrogen).

Cell - Specific Targeting Strategies for Electroporation - Mediated Gene Delivery in Cells and Animals.

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

C, Typical number of iPSC colonies obtained with electroporation of pCEP - Oct4 and Nanog or with Oct4 alone.

HV cells overexpressing Nanog were generated by electroporation with a vector containing the Nanog cDNA under the control of a CAG promoter and upstream of IRES Puro cassette followed by selection in puromycin (2 µg / ml) for 2 wk.

The repository of available techniques includes microinjection, particle bombardment («gene gun» technology), laser irradiation, electroporation, sonoporation, and magnetofection (Table 1).

Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use electroporation to ensure a good transformation efficiency.

1 - 4 days following DNA injection and electroporation, embryonic brains are dissected, sliced, placed on a filter in culture medium and live imaged over night (Baffet et al., Methods Cell Biol., 2015).

For a typical library, Addgene usually does four to eight electroporation reactions using a total of ~ 400ng of library DNA.

The electroporation treatment produced no toxicity and no serious adverse events, aside from injection site pain (69.0 %) and inflammation (20.7 %).

Electroporation of investigator - provided construct into mouse embryonic stem cells / Service Request form plus documentation.

With intratumoral electroporation, tumor cell pores are opened when a mild electrical current is introduced directly through an electrode.

Systemic antitumor effect and clinical response in a phase 2 trial of intratumoral electroporation of plasmid interleukin - 12 in patients with advanced melanoma.

Interim phase II results [12] of first treatment with electroporation of IL - 12 in 28 patients with advanced melanoma, after 24 weeks of treatment, reported at the American Society of Clinical Oncology 2014 Annual Meeting, revealed a 32.2 % objective response rate (ORR; the primary endpoint), with a CR in 10.7 %.

In this study we review the most commonly used indel detection methods and using a robust, sensitive, and cost efficient Indel Detection by Amplicon Analysis method, we have investigated the impact of the most commonly used CRISPR / Cas9 delivery formats, including lentivirus transduction, plasmid lipofection, and ribo nuclear protein electroporation, on the dynamics of indel profile formation.

Electroporation of investigator - provided construct with 10 - 15 colonies picked for analysis / Service Request form.

Additionally, the authors tested for cellular specificity by using as a convenient experimental framework in utero electroporation of a plasmid that allowed co-expression of the PLE enzyme and of a fluorescent tag (mCherry).