Sentences with phrase «eb formation»

In the case of EB formation using mouse testicular or ovarian cell - conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned medium.

In monkeys, methods for inducing germ cell differentiation from ES cells have not been reported except spontaneous germ cell differentiation by EB formation [7].

In humans, germ cell differentiation from ES cells via spontaneous EB formation, and EB formation with recombinant human bone morphogenetic proteins (BMPs) has been reported [5], [6].

However, most EB formation protocols contain undefined components, such as Fetal Bovine Serum (FBS), Knock - Out Serum Replacement (KOSR) or albumin product.

As such, it is essential that EB formation and further differentiation can be conducted in chemically defined, animal product - free conditions.

Not exact matches

This process (see figure) started with spin - embryoid body (spin - EB) formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.

To induce ectoderm formation, EBs were cultured in DMEM / F12 and 1 % N2 supplemented with retinoic acid (2 uM) and forskolin (5 uM).

Although no morphological features that suggested the formation of follicle - like structures were observed in EBs as reported in mice [4], these results suggest that the conditioned medium induce VASA and SCP1 expression in monkeys.