Sentences with phrase «es cell clones»

The investigator is responsible for demonstrating a reliable scheme for identifying targeted ES cell clones by Southern analysis with 5» and 3» probes by providing results from pilot experiments for this purpose.

However, incorrectly identified ES cell clones are occasionally encountered, either because of human error or because a thawed ES cell line gets overgrown by a contaminating ES cell line.

Large scale sequencing of ES cell clones was conducted and sequence tags have been deposited to NCBI's dbGSS.

IKMC ES cell clones that are assigned for mouse production in IMPC (www.mousephenotype.org) will not be con sidered by the panel.

ES cells must be sourced by applicants from ES cell repositories and projects will only be initiated if two independent quality controlled ES cell clones are available

2) If ES cell clones are not available from the IKMC / IMPC resource, mouse models will be generated using genome editing approaches such as CRISPR / Cas9 nuclease technology (on C57BL / 6N genetic background only) and may cover constitutive knock - outs or point mutations.

Through special agreements with originators, the MMRRC distributes mutant ES cell clones, parental and feeder cell lines.

The following methods can be used to assure the identity of the ES cell clone:

An expansion of an ES cell clone is a minimal service and does not include verification of the identity of the ES cell line by DNA sequencing.

The service provided covers the production of a minimum of two heterozygous mice carrying the targeted gene allele of choice from the corresponding validated gene - targeted ES cell clone (s).

The vast majority of BayGenomics cell lines have been correctly identified, as judged by DNA sequencing (i.e., repeat determination of the DNA sequence tag when the ES cell clone is requested).

Not exact matches

The images are meant to show that the ES cells from cloned embryos look similar to those derived from IVF embryos, suggesting the cloned ES cells are the real thing.

He says that he is ready to ship the cloned ES cell lines to several labs that have requested them as soon as the Oregon institutional review board signs off on the transfers, which could happen in a matter of days.

Although scientists have welcomed the development, they say the real breakthrough will be when someone manages to extract ES cells from the inner cell mass of cloned blastocysts and generate a cell line from them.

Many scientists argue that so - called research cloning, in which cloned human embryos might be used to produce embryonic stem (ES) cells, could be a boon to medicine.

If the cells can be fertilized and develop into viable embryos, and if human ES cells turn out to have similar powers, such cells could allow researchers to get around some of the expense and ethical questions that arise from using donated eggs for therapeutic cloning experiments.

No one has yet shown success with SCNT using human tissue: The closest effort so far has been the cloned blastocyst reported last year by workers at the Newcastle Fertility Centre in the U.K., but that did not yield ES cells.

With so much work needed in studying the nature of stem cells and using them to study disease processes, therapies based on ES cells seem very far down the line, noted Lorenz Studer of Memorial Sloan - Kettering Cancer Center in New York, who pointed out that so far there have only been two published papers on therapeutic cloning, both of them in mice.

It won support in spite of two previous House votes to outlaw all forms of cloning, including research cloning (otherwise known as nuclear transfer), which scientists say is essential to realize the promise of ES cell research.

Moretta A, Vitale M, Bottino C, Orengo AM, Morelli L, Augugliaro R, Barbaresi M, Ciccone E, Moretta L. P58 molecules as putative receptors for major histocompatibility complex (MHC) class I molecules in human natural killer (NK) cells: anti-p58 antibodies reconstitute lysis of MHC class I - protected cells in NK clones displaying different specificities.

If no ES cells or less than 3 clones are available within the IKMC consortium, only the constitutive knock - out model will be generated using CRISPR genome editing technology.

ES cells were targeted and hygromycin resistant clones screened by Southern blot.

Error bars represent standard deviation between expression levels in independent clones of HV ES cells.

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

ES cells from two HV clones (5.1 and 16.1) were used to generate chimeras by morula aggregation.