Sentences with phrase «es cell colonies»

To produce EBs, ES cell colonies were removed from feeder cells using a detaching solution, which consisted of 0.25 % trypsin supplemented with 1 mg / ml collagenase IV, 1 mM CaCl2, and 20 % knockout serum replacement (KSR; Invitrogen, Carlsbad, CA).

Not exact matches

Although some teams have managed to produce nearly pure colonies of certain kinds of neural cells from ES cells, no one has managed to concoct a recipe that will direct the cells to become, say, a pure population of dopamine - producing neurons that could replace those missing in Parkinson's disease.

A few months ago, researchers reported that oocytes can form spontaneously in colonies of mouse ES cells (ScienceNOW, 1 May), the first demonstration that ES cells can form germ cells in vitro.

Activities concerning DNA targetting vectors construction, mouse ES cells generation, genetically modified mice generation, mice colony housing, breeding and maintenance under respect of ethical rules and animal welfare, rederivation, cryorecovery and cryopreservation services., have been certified ISO 9001:2015.

Following this scheme, ES cell - like colonies appeared gradually and were picked up on day twelve.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

E, Established human iPSC colonies, with well - defined borders and compact cells, are morphologically similar to hESCs.

We sought first to examine gene expression in single ES cells isolated directly from specific zones within colonies, with two objectives.

(A) Expression of 5 stem marker genes in single cells isolated from the edge (E), mid (M) or adjacent center (C) region of HES2 colonies.

Briefly, ES colonies were maintained on mitotically inactivated mouse embryonic fibroblasts (density of 60,000 cells / cm2) in media consisting of DMEM (Invitrogen cat.

Single cell Q - RTPCR for the genes indicated was carried out on 87 cells isolated from different regions of ES colonies.

Peerani et al. [31] suggested that there is a minimal colony size effect the maintenance of human ES cell pluripotency, and provided evidence that autocrine regulators of BMP and nodal signaling are key components of this regulatory pathway.

The flow cytometry results showed a quantitative continuous gradient of stem cell antigen expression in ES cell cultures, a gradient that reflected the position of cells in a colony relative to the feeder cell layer.

(E) Rat1 - DD cells expressing kinase - inactive alleles of MELK (D150A or T167A), fail to grow as colonies in soft agar.