Not exact matches
The
fraction of F4 / 80 - expressing
cells was greater in moderately obese mice (b and
e) and greatest in the severely obese Lepob / ob mice (c and f).
In a similar way, we used HV lacZ
ES cells to ask whether the V+S +
fraction would preferentially segregate to the outside of chimeric EBs.
With this reporter we split
ES cell cultures into two
fractions that both express certain stem
cell markers but only one of which expresses low levels of an endodermal marker gene.
Most of these genes were significantly down - regulated in both V +
fractions and remain high in the V − S −
fraction, indicating that this
fraction contained a significant proportion of undifferentiated
ES cells.
Initially we injected purified
fractions of HV
ES cells into Rosa26 blastocysts that constitutively express β - galactosidase (β - gal) and examined embryos at 9.5 dpc for
ES cell (β - gal negative) contribution (Table S4 and Figure S5).
HV
ES cells grown under self - renewing conditions were fractionated by flow cytometry into four
fractions based on Venus (V) and SSEA - 1 (S) expression.
(C) Measurement of Phospho - Erk levels in V+S + and V − S +
fractions shows an enrichment of activated Erk with Venus positive
ES cells.
We show that the
fraction of
cells present within this state is influenced by factors that both promote and suppress primitive endoderm differentiation, but conditions that support
ES cell self - renewal prevent their progression into differentiation and support an equilibrium between this state and at least one other that resembles the Nanog positive inner
cell mass of the mammalian blastocysts.