Sentences with phrase «es cell line»

Ordering more than one ES cell line for a specific «gene - of - interest» will increase the chances of success.

The Sanger Institute Gene Trap Resource (SIGTR) mouse ES cell line collection was developed to provide a powerful and cost effective approach using gene trapping to create large numbers of insertional mutations that are immediately accessible to molecular characterization.

We also recommend that recipients of ES cells re-verify the identity of the ES cell line by RT - PCR and / or Southern blot analysis (Genotyping BayGenomics Mice).

Therefore, we strongly recommend that you request verification of the identity of your ES cell line by DNA sequencing.

However, incorrectly identified ES cell clones are occasionally encountered, either because of human error or because a thawed ES cell line gets overgrown by a contaminating ES cell line.

We use an ES cell line in which low levels of Hex transcript are visualized based on the expression of the enhanced YFP, Venus coupled to a unique translational amplifier.

A cynomolgus monkey ES cell line, CMK6, was cultured as previously described [14].

As noted in the JAX ® Mice strain data sheets, the best control for a targeted mutant is the 129 strain used to produce the ES cell line from which the mutant was derived (Simpson et al. 1997; Threadgill et al. 1997).

The UC06 (HSF6) human ES cell line (P62) was obtained from the National Stem Cell Bank (NSCB), and the adipose derived mesenchymal stem cell (AD - MSC) line was generously obtained from Dr. Jeffrey Gimble from the Penington Biomedical Research Institute.

On further culture or after subcloning this ES cell line at various times (passages), this particular mtDNA haplotype showed essentially random drift, with cells carrying anything from 0 to 100 %.

This result was similar to recently published data from the Egli lab in New York, which used an alternative technique, that of maternal spindle transfer or MST in eggs prior to fertilization, where they also had one aberrant ES cell line where this occurred (out of 8).

• ES cell line purity verification In addition to mouse strain purity verification, the facility provides genetic screening of ES cell lines used for gene targeting experiments.

For both BayGenomics parental ES cell line E14Tg2a.4 (also used for Sanger (SIGTR) lines) and Soriano parental ES cell line AK7, ultra low passage cells suitable for multiple electroporations and germline transmission are available.

An expansion of an ES cell clone is a minimal service and does not include verification of the identity of the ES cell line by DNA sequencing.

The team makes it clear that it thinks its approach is superior to one reported last month in Nature by scientists at Advanced Cell Technology (ACT) in California — deriving an ES cell line from a single cell taken from a morula (ScienceNOW 23 August).

Where permitted, ES cells should be drawn from sources in the following order: (1) existing ES cell lines, originating from ES cells derived from embryos less than 14 days old; and (2) surplus human embryos less than 14 days old that were created for fertility treatment.

He says that he is ready to ship the cloned ES cell lines to several labs that have requested them as soon as the Oregon institutional review board signs off on the transfers, which could happen in a matter of days.

In a paper published online yesterday in Stem Cells, the researchers report that they succeeded in generating pluripotent human ES cell lines — i.e., cells that can develop into many different kinds of cells — from one of the 13 late - arrested embryos.

The earlier - arrested embryos did not produce ES cell lines.

The final guidelines on research with human embryonic stem cells issued on Monday by the National Institutes of Health set out criteria for determining which ES cell lines can be used in federally funded experiments and give NIH discretion to approve old lines that don't meet stringent modern ethical requirements.

Although the president's decision may temporarily halt a political push for more research opportunities, his assertion that some 60 ES cell lines would be available for research is more than twice the number most scientists would cite.

Since last year, teams in Italy, Scotland, and the United States have reported deriving ES cell lines from human parthenotes, although none of the lines is well studied.

An international team led by researchers at Johns Hopkins University in Baltimore, Maryland, compared early - passage and late - passage versions of nine human ES cells lines obtained from labs around the world.

Nonetheless, passage of the measure marks a significant milestone in efforts to overturn the Bush policy, which limits government funding to research using 60 human ES cell lines derived before 9 August, 2001.

Three levels of parental ES cell lines are available for E14Tg2a.4 (BayGenomics and Sanger Institute Gene Trap Resource (SIGTR)-RRB- and AK7 (Soriano) cell line collections; only Level 2 of parental ES cell line is available for the Nagy collection - see item 2 below:

He is part of the pioneering groups which established human ES cell lines and distributed them to other scientists free of charge.

Fluorescent transgenic ES cell lines - PCR assays are provided for the marker transgene.

The Repository of mouse ES cell lines, strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

He added: «Truly naive human ES cell lines would not only help answer fundamental questions about how we are made, and be useful for drug screening and tissue therapy, but they would also provide a benchmark against which other types of stem cells could be measured in terms of their effectiveness in stem cell therapy and regenerative medicine.»

«Seven out of eight ES cell lines derived from (parthenogenetic) embryos after MRT (spindle transfer) showed no or very low persistence of the mtDNA haplotype that was carried over with the nuclear DNA.

Flpe - and Cre - mediated recombination in vitro generated ES cell lines that contained a conditional or null Sema3B allele, respectively, which were established as homozygous alleles in mice.

Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129 - derived ES cell lines.

Here we show that DNA methylation is globally reduced in XX ES cell lines and that this is attributable to the presence of two active X chromosomes.

However, the levels of this transcript are presumably extremely low as they were not detected in fluorescent Hex reporter ES cell lines [30].

This study focuses on the engraftment of HPCs in immunocompetent mice and proposes a strategy that could exploit available human ES cell lines to improve organ transplantation.

Comparison of gene expression profiles showed a low number of differentially expressed transcripts among the three ES cell lines.

The MMRRC currently holds 24 mouse models and several ES cell lines from this collection and the MMRRC at UC Davis will generate an additional group of mouse models for this collection from the supplied ES cells.

Figure S1 shows QRT - PCR data for expression of the TGF - beta superfamily member GDF3, a gene which is strongly downregulated across the various subpopulations, for four ES cell lines subjected to separation by flow cytometry.

Most, but not all BayGenomics ES cell lines yield germline - transmitting chimeras.

The European Mouse Mutant Cell Repository (EuMMCR) holds and distributes vectors and mutant ES cell lines produced in the EUCOMM and EUCOMMTools consortia representing over 16.800 different genes.

FRS, and William Skarnes, Ph.D., at the Wellcome Trust Sanger Institute, plans to systematically create mouse ES cell lines in which 5,000 genes have been knocked out by gene targeting.

This collection has approximately 58 ES cell lines and 44 mouse lines archived.

The majority (but not all) of the BayGenomics ES cell lines were generated from 129P2 / OlaHsd (formerly 129 / Ola) ES cell lines, predominantly the E14Tg2A.4 parental subclone.

Users of SIGTR ES cell lines are advised to follow the protocols available on the SIGTR website.

Gene expression profiles are provided for all RNA samples combined, or separately for the CGR8 and E14TG2a ES cell lines.

QRT - PCR analysis of GDF - 3 expression in immunologically defined subpopulations of four different human ES cell lines.

The Mutant Mouse Resource and Research Center (MMRRC) distributes and cryopreserves scientifically valuable, genetically engineered mouse strains and mouse ES cell lines with...

Not exact matches

That summer, President George W. Bush had approved federal funding for human ES - cell research, but only for a small number of cell lines that had already been created.

The figure in question is supposed to show patterns of expression for a range of ES cell markers in the 11 cell lines.

In May 2005, Hwang and his colleagues reported that it had produced 11 new human embryonic stem (ES) cell lines that carried the genetic signature of patients with diabetes, spinal cord injury, or a genetic blood disorder (Science, 20 May, p. 1096).