Sentences with phrase «es cell markers»

Venus positive cells experiencing low - level transcription at the Hex locus, but still expressing the ES cell markers SSEA - 1 and Oct4, show elevated levels of PrEn gene expression and reduced levels of early ICM markers such as Nanog.

Using this cell line we show that apparently undifferentiated ES cell cultures consist of at least three cell types defined by this lineage - specific low - level transcription and the expression of the ES cell markers Oct4 and Nanog.

The figure in question is supposed to show patterns of expression for a range of ES cell markers in the 11 cell lines.

One of these cells types, characterized by both the expression of the Venus transgene and the ES cells marker SSEA - 1 (V+S +), appears to represent an early step in primitive endoderm specification.

Not exact matches

Fluorescent transgenic ES cell lines - PCR assays are provided for the marker transgene.

The scientists also discovered that genes modified only by one of the epigenetic markers, H3K4me3, contain the DNA recipes for proteins that enable an ES cell to proliferate, or duplicate itself.

Determining how ES cell genes are modified by these epigenetic markers may explain these cells» unique characteristics, said the scientists, who are based at the Genome Institute of Singapore (GIS) and the Bioprocessing Technology Institute (BTI), both under the Agency for Science, Technology and Research (A * STAR), as well as at the National University of Singapore (NUS).

Therefore, VASA is a potential marker for ES cell - derived germ cells in monkeys.

PRDM14 is required for the development of mouse germ cells [17], and also known as a pluripotent marker for mouse and human ES cells [17], [52].

However, SSEA1 can be a useful cell surface marker for the detection and enrichment of putative germ cells derived from ES cells as reported previously [56].

With this reporter we split ES cell cultures into two fractions that both express certain stem cell markers but only one of which expresses low levels of an endodermal marker gene.

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

Recent observations suggest that there may be lineage - specific markers expressed in sub-populations of ES cell cultures.

The expression of germ cell marker genes in monkey ES cells and tissues was examined using an RT - PCR analysis.

With regard to the meiotic germ cell markers, SCP1 and SCP3 expression was also detected in the testes, whereas MLH1 was expressed in various tissues and ES cells.

Among the early germ cell markers examined, VASA is a candidate gene for detecting pre-meiotic germ cell differentiation from monkey ES cells, because its expression is detected earlier in the primordial stage of germ cell development in comparison to that of PIWI family genes in vivo in mice and humans [11], [36]--[38], [49].

This observation suggests that PIWIL1 could be a marker gene for the detection of ES cell - derived germ cells.

VASA is a valuable gene for the detection of germ cells differentiated from ES cells in monkeys, and the identification and characterization of germ cells derived from ES cells are possible by using reported germ cell markers in vivo, including SSEA1, OCT - 4, and VASA, in vitro as well as in vivo.

However, while these markers are useful tools, ES cells can only be defined based on retrospective function.

However, these germ cell marker genes are not appropriate for detecting germ cell differentiation from mouse and human ES cells because these genes are expressed in both ES cells and germ cells.

Therefore, SCP1 and SCP3 were employed as markers to detect the meiotic stage in ES cell - derived germ cells.

A combination of several germ cell markers will thus be necessary to detect germ cell differentiation from ES cells.

To explore the differentiation markers for detecting germ cells differentiated from ES cells, the expression of various germ cell marker genes was examined in tissues and ES cells of the cynomolgus monkey (Macaca fascicularis).

SSEA1 is a possible marker to detect ES cell - derived germ cells in monkeys, since its expression is found not only in primordial germ cells in vivo [55] but also in ES cell - derived germ cells in vitro in mice and humans [3], [56].

Three recent experimental studies focused on low consumption / exposure.949596 In one study, 29 smokers each consumed a single cigarette, immediately after which they had a significant decrease in blood vessel output power and significant increase in blood vessel ageing level and remaining blood volume 25 minutes later, as markers of atherosclerosis.94 In another study, human coronary artery endothelial cells were exposed to the smoke equivalent to one cigarette, which led to activation of oxidant stress sensing transcription factor NFR2 and up - regulation of cytochrome p450, considered to have a role in the development of heart disease.95 These effects were not seen when heart cells were exposed to the vapour from one e - cigarette.95 A study exposed adult mice to low intensity tobacco smoke (two cigarettes) for one to two months and found adverse histopathological effects on brain cells.96

CXCR4, which is a chemokine receptor, is expressed in migratory and post-migratory germ cells in vivo in mice [27], and is also a marker for germ cells derived from human ES cells [28], thus suggesting that CXCR4 is a candidate marker gene for detecting ES cells - derived germ cell in monkeys.

(A) The expression patterns of germ cell marker genes in monkey testis (5 years old), mouse embryonic fibroblast (MEF), monkey ES cells (ES), and developing EBs (days 3, 7, 14, 21, and 28) were examined using an RT - PCR analysis.

ES cells can be described based on a characteristic morphology, the presence of cell surface markers such as SSEA - 1 and Pecam1, or the expression of the key transcription factors such as Oct4, Sox2, Nanog, and a number of ES cell - specific transcripts (ECATs)[4]--[6].

(A) Expression of 5 stem marker genes in single cells isolated from the edge (E), mid (M) or adjacent center (C) region of HES2 colonies.

Here, ES cells transduced with HOXB4 were analyzed for the expression of hematopoietic markers after 26 days of differentiation.

(E) Rhodopsin - positive material (red) is present within the cell membrane of human specific marker (HSM)- labelled iPS - RPE (green) 8 days post-transplantation and in the tips of the host outer segment (OS) layer.

(E) Western blot analysis of iPS - RPE protein expression using antibodies against a panel of RPE cell markers.

Our laboratory used two monoclonal antibodies, GCTM - 2 and TG30, recognizing the cell surface proteoglycan characteristic of primate ES cells and CD9 respectively, to fractionate human ES cell populations into four compartments according to their relative levels of expression of both markers [14].

These iPS cells have a normal karyotype, demonstrate telomerase activity and express embryonic stem (ES) cell surface markers.

Barbareschi M, Pecciarini L, Cangi MG, Macri E, Rizzo A, Viale G, Doglioni C. p63, a p53 homologue, is a selective nuclear marker of myoepithelial cells of the human breast.

Germ cell specific markers are also expressed in embryoid bodies derived from human ES suggesting the presence of PGC's, so I am developing methods to enhance their formation and isolation.

(E) Cyp7b1 + / − or Cyp7b1 − / − recipients were reconstituted with WT or lymphotoxin (LTβ10) transgenic (tg) BM cells and analyzed by flow cytometry for the indicated markers.

Closely monitoring several markers of cell damage (including creatine kinase, lactate dehydrogenase, prostaglandin - E and tumor necrosis factor - alpha) in their sample of 18 male athletes (who used 20 grams of creatine monohydrate per day for five days, mixed with 60 grams of maltodextrine), the researchers found levels of these markers were reduced after the race, compared to 16 control subjects who took only the maltodextrine.