Sentences with phrase «es cell subpopulations»
HV cells cultured under self - renewing conditions were subjected to flow cytometry to separate Venus positive and negative ES cell subpopulations and injected into Rosa26 LacZ expressing blastocysts within 1 h of purification.
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Does this heterogeneity define a functional subpopulation of cells in ES cell cultures?
(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.
Figure S1 shows QRT - PCR data for expression of the TGF - beta superfamily member GDF3, a gene which is strongly downregulated across the various subpopulations, for four ES cell lines subjected to separation by flow cytometry.
QRT - PCR analysis of GDF - 3 expression in immunologically defined subpopulations of four different human ES cell lines.