Sentences with phrase «es cells in culture»

In a study published earlier this year, the same Belmonte and Gage lab team demonstrated that a few ES cells in a culture dish tended to lose stemness and evolve into muscle cell precursors, most likely goaded by a muscle differentiation factor known as BMP.

«Using this cell line allows us to separate 2 - cell - like cells from the ES cells in the culture by collecting the green cells which have entered the 2 - cell like state.

Not exact matches

In a culture of embryonic stem (ES) cells, a small population (around 1 %) spontaneously turns into cells that are similar to the totipotent cells of the 2 - cell stage embryo.

This enables robust stem cell culture methods that in practical solves all current technical challenges with culturing human ES and iPS cells.

Human ES and iPS cells grow as monolayers in a completely defined and xeno - free cell culture environment that enables self - renewal and pluripotency without the use of ROCK inhibitors.

A future aspect of our mouse work is directed towards use of ES cell differentiation in culture as a model for epigenetic decisions and stem cell manipulations.

Studies in cultured embryonic stem (ES) cells, and our analysis of zebrafish embryos have revealed that pluripotency is characterized by a unique chromatin signature.

In light of the observation that iPS cell derivation takes place under the same culture conditions used for ES cells [20], we hypothesized that these human feeder cells could offer a stable tool for defining molecular hallmarks during conversion of differentiated somatic cells to the pluripotent state.

In addition, their independence of LIF for iPS cell culture provides an opportunity to further understand the ground state of ES cell self - renewal proposed by Ying et al. [21].

Our unpublished data show that HFF cells can support derivation and culture of mouse ES cells in an undifferentiated state without exogenous LIF for more than 30 passages.

(C) When iPS cells were cultured in the absence of the HFF cells, various differentiated cells could be observed, such as definitive endoderm - like cells (a) and (b), fibroblast - like cells (c), cardiomyocytes (d), epidermis - like cells (e) and neural rosettes (f).

The low levels of Hex transcript observed in undifferentiated ES cells (Figure S2C) were sufficient to generate a significant Venus positive (V +) sub-population in undifferentiated ES cell cultures grown under standard feeder free conditions.

Thus while the majority of the V + population existing in ES cell cultures are indistinguishable from undifferentiated ES cells, we also observe differentiated cells expressing high levels of the Venus transgene (arrows in Figure 2B) that resemble the high - level Venus expressors generated in response to differentiation and that probably represent spontaneous PrEn differentiation.

Does this heterogeneity define a functional subpopulation of cells in ES cell cultures?

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

In contrast, the same mouse ES cells cultured on MEF cells began to differentiate after 2 passages in the absence of LIF (data not shownIn contrast, the same mouse ES cells cultured on MEF cells began to differentiate after 2 passages in the absence of LIF (data not shownin the absence of LIF (data not shown).

(A) Flow cytometry of two independent HV clones (HV 5.1 and HV 16.1) cultured either under self - renewing conditions or in the absence of LIF show the presence of a subpopulation of cells positive for Venus and / or the ES cell surface marker SSEA - 1.

Recent observations suggest that there may be lineage - specific markers expressed in sub-populations of ES cell cultures.

Nanog overexpressing and control cell lines were cultured in the absence of LIF for 10 d and assessed for ES cell - like morphology.

In addition to expressing slightly increased PrEn gene expression, V+S + cells also contain almost all the phospho - ERK activity in our ES cell cultures (Figure 6In addition to expressing slightly increased PrEn gene expression, V+S + cells also contain almost all the phospho - ERK activity in our ES cell cultures (Figure 6in our ES cell cultures (Figure 6).

Taken together our data support a model in which ES cell culture has trapped a set of interconvertible cell states reminiscent of the early stages in blastocyst differentiation that may exist only transiently in the early embryo.

ES cells were differentiated toward ADE in aggregation culture according to [30].

However, when maintained in ES cell culture, cells transit between these states.

In the case of EB formation using mouse testicular or ovarian cell - conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned mediuIn the case of EB formation using mouse testicular or ovarian cell - conditioned medium (see below), the EBs were cultured in ES medium for 24 hr, and then in a conditioned mediuin ES medium for 24 hr, and then in a conditioned mediuin a conditioned medium.

It was recently suggested that Hes1 expression can cycle in ES cell culture [13], although the link between this oscillation, low - level gene expression, and developmental bias is not clear.

While Hex is discretely expressed in the VE on the anterior side of the embryo, it is initially expressed throughout the early PrEn [28] and like the GATA factors, Hex transcripts are also detectable in some ES cell cultures [29].

In addition to feeder free serum and LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42In addition to feeder free serum and LIF containing media, ES cells can be cultured in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42in minimal serum free media (referred to as 2i) containing the MEK inhibitor PD0325901 that targets the phospho ERK branch of the FGF pathway and the GSK3 - β antagonist CHIR99021 [42].

Cultures of unfixed ES cells were sorted into four populations for parallel analysis of gene expression and development in cell culture.

ES cells were maintained in culture according to standard conditions as we have previously described in detail [16], [26].

Differentiation into extraembryonic endoderm [21] or neural progenitors [33] are frequent early outcomes of spontaneous differentiation when human ES cells are cultured in the presence of a feeder cell layer, and it is interesting to speculate that the cells are being primed for these fates.

Heterogeneity in human ES cultures is reflected by the variability in expression of cell surface antigens seen under culture conditions that promote stem cell renewal.

Lineage commitment in the mammalian embryo is most often depicted as a series of binary choices between alternate cell states, and increasing evidence supports the hypothesis that fate decisions in embryonic stem (ES) cell cultures reflect these developmental processes [1].

«It was exciting because nobody knew that «reverse differentiation» occurred in ES cell cultures,» he said referring to the first report.

The flow cytometry results showed a quantitative continuous gradient of stem cell antigen expression in ES cell cultures, a gradient that reflected the position of cells in a colony relative to the feeder cell layer.

Dienogest, a synthetic progestin, inhibits prostaglandin E (2) production and aromatase expression by human endometrial epithelial cells in a spheroid culture system..