For
embryoid body formation, iPS cells were seeded into ultra low attachment plates (Corning) containing DMEM + 20 % FBS.
Undifferentiated embryonic stem (ES) cells express Oct4 mRNA and protein; this expression is rapidly downregulated during
embryoid body formation.
Not exact matches
This process (see figure) started with spin -
embryoid body (spin - EB)
formation from cultures of single - cell hiPSC suspensions in 96 well plates, and the subsequent addition of BMP4 and FGF - 2 to induce mesoderm differentiation, and then VEGF and SCF to induce hematopoietic differentiation.
BMP4 Promotes
Formation of Primitive Vascular Networks in Human Embryonic Stem Cell — Derived
Embryoid Bodies.
Germ cell specific markers are also expressed in
embryoid bodies derived from human ES suggesting the presence of PGC's, so I am developing methods to enhance their
formation and isolation.