Sentences with phrase «embryonic cell and gene»

Mitalipov is director of the Center for Embryonic Cell and Gene Therapy at OHSU.

On Aug. 3, the scientific article in Nature finally gave us some facts about the much - hyped experiments that involved editing the genomes of human embryos at the Center for Embryonic Cell and Gene Therapy at Oregon Health and Science University.

Not exact matches

In SIF - seq, hundreds or thousands of DNA fragments to be tested for enhancer activity are coupled to a reporter gene and targeted into a single, reproducible site in embryonic cell genomes.

Base oxidation regulates gene activity In cooperation with colleagues at LMU, as well as researchers based in Berlin, Basel and Utrecht, Carell and his group have now shown, for the first time, that a standard base other than cytosine is also modified in embryonic stem cells of mice.

The stem cells, derived from human umbilical cord - blood and coaxed into an embryonic - like state, were grown without the conventional use of viruses, which can mutate genes and initiate cancers, according to the scientists.

The team also found that ERAS, a tumorigenic gene expressed in mouse embryonic stem cells and iPSCs, was mutated and dysfunctional in the mole - rat iPSCs.

Further investigation, says Resar, showed that these unusual properties arise from the ability of HMGA1 to turn on several genes involved in the Wnt pathway, a network of proteins necessary for embryonic development and stem cell activity.

Early in embryonic development, both mouse and human placentas rely on the same set of ancient cell - growth genes.

When Kaufman, Zon and colleagues looked to see what was different about these early cancer cells, they found that crestin and the other activated genes are the same ones turned on during zebrafish embryonic development — specifically, in the stem cells that give rise to the pigment cells known as melanocytes, within a structure called the neural crest.

This program normally shuts off after embryonic development, but occasionally — for reasons not yet known — crestin and other genes in the program turn back on in certain cells.

Although Oct4 and Sox2 were well - known players in embryonic cells, «we thought this would be such a complicated problem, we never tested those genes up front,» Thomson says.

Yamanaka's group and two others followed up earlier this year with firmer evidence that these induced pluripotent stem (iPS) cells faithfully mimicked the patterns of gene activity and cellular differentiation observed in embryonic stem cells.

They propose that normal tissue becomes primed for cancer when oncogenes are activated and tumor suppressor genes are silenced or lost, but that cancer develops only when a cell in the tissue reverts to a more primitive, embryonic state and starts dividing.

As part of this effort, Dr. Weinstein and his colleagues inserted a gene for a protein that turns green the cells that line the endothelium of selected embryonic veins and in the lymphatic system — the network of vessels through which immune cells travel in the body.

Wondering why the third protein, an enzyme called p66, was not, despite being very similar to the other two, Pelicci's team knocked out the piece of the gene that enabled it to code for p66, in order to make mice and mouse embryonic cells that lacked p66.

Derived mostly from human embryonic kidney 293T (HEK293T) and HeLa cell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gcell lines, EdiGene Knockout (KO) Cell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gCell Lysates have been optimized through the use of genome editing technology and validated at the genomic level through PCR and Sanger - sequencing techniques to ensure the accuracy and knockout of the target gene.

The results point to indirect genomic regulatory mechanisms which are important for embryonic stem cells and maintain gene expression, say Riikka Lund and Riitta Lahesmaa.

This finding contradicts the field's presumptions about this important gene and its role in the differentiation of embryonic stem cells.

- Our results provide new insights into the mechanisms of how POLR3G gene regulates stem cell state, which in turn sheds light on the complex mechanisms with which human embryonic stem cells both self - renew and maintain the ability to differentiate.

Mitchell and her colleagues eliminated this possibility when they deleted these nearby regions in the genome of mice and found there was no impact on the gene's ability to be turned on in embryonic stem cells.

A large body of research has reported that Nanog is allelically regulated — that is, only one copy of the gene is expressed at any given time — and fluctuations in its expression are responsible for the differences seen in individual embryonic stem (ES) cells» predilection to differentiate into more specialized cells.

«We studied how the Sox2 gene is turned on in mice, and found the region of the genome that is needed to turn the gene on in embryonic stem cells,» said Professor Jennifer Mitchell of U of T's Department of Cell and Systems Biology, lead invesigator of a study published in the December 15 issue of Genes & Development.

Thorough study of changes in the gene activity regulation mechanism showed that reprogrammed and embryonic stem cells are similar.

And so they have long sought haploid embryonic stem cells, which can become any kind of tissue but contain just one set of genes, like a sperm or egg.

But when physiologist H. Lee Sweeney of the University of Pennsylvania School of Medicine in Philadelphia and his colleagues put this faulty gene into embryonic quail muscle cells growing in lab dishes, the cells made a shortened version of the protein and incorporated it into their contractile machinery.

Three teams of scientists reported earlier this year that they had directly reprogrammed adult mouse skin cells into embryonic cells, although the process involved viruses and cancer - causing genes.

Schöler and his team looked at embryonic mouse clones that were just a few days old to see when and where the Oct4 gene — which helps embryonic cells decide where to go and what to do — is active.

IPSC's are derived from the donated skin or blood cells of adults and, with the reactivation of four genes, are reprogrammed back to an embryonic stem cell - like state.

Not only did a high percentage of embryonic cells get repaired, but also gene correction didn't induce any detectable off - target mutations and genome instability — major concerns for gene editing.

In order to find the genes that guide that migration, geneticist Ruth Lehmann of the Howard Hughes Medical Institute at New York University Medical School and her colleagues used chemicals to cause mutations in thousands of adult fruit flies then screened their embryonic offspring for lost or misguided germ cells.

They have learned how to turn on and off essential genes and how to transform embryonic stem cells into retinal cells, which can be transplanted into mice to restore vision.

They hope to identify the complete set of DNA binding sites and corresponding target genes for the regulons in embryonic stem cells and a subset of the cells they differentiate into.

By transferring the gene for melanopsin into human embryonic kidney cells, synthetic biologist Martin Fussenegger of the Swiss Federal Institute of Technology in Zurich and colleagues made these cells light - sensitive as well.

The researchers took skin cells from the tails of sickle cell mice and inserted copies of four genes that made the cells take on the characteristics of embryonic stem cells.

Tumors without that gene became much less differentiated and more similar to embryonic cells.

The mouse embryonic stem cell knockout resource provides a basis for the characterization of relationships between genes and phenotypes.

It turns out that iPS cells and embryonic stem cells have differing patterns of methylation, a modification of DNA that can alter how genes behave even if the underlying DNA sequence remains the same.

The Mouse Molecular Technologies team provide high - throughput genotyping and characterisation of mutant mouse strains created either by targeted embryonic stem cells (ESC) or CRISPR / Cas9 gene editing in single - cell zygotes.

Neuronal developmental gene and miRNA signatures induced by histone deacetylase inhibitors in human embryonic stem cells.

A further 25 days in medium containing FGF2 and Noggin mediated the derivation and expansion of RPCs which had a similar global gene expression pattern to cells of the embryonic day 14 (E14) mouse retina.

In the new research, Pollen and co-first author Tomasz Nowakowski, PhD, also a postdoctoral researcher in the Kriegstein lab, partnered with Fluidigm Corp. to develop a microfluidic approach to map out the transcriptional profile — the set of genes that are actively producing RNA — of cells collected from the VZ and SVZ during embryonic development.

Yamanaka and Takahashi began their search by studying embryonic stem cells in the hope of identifying the genes that underlie essential stem cell characteristics, such as pluripotency and proliferation, a cell's ability to replicate itself.

It concentrates on analysis of gene function at all levels: the gene and its product, the cell and cellular interactions, embryonic tissues and the entire animal.

Probably a number of embryonic genes, after being turned off for decades, are reexpressed in cancer cells, enabling those cells to regain their embryonic capacity to move around and invade other tissues.»

The Repository of mouse ES cell lines, strains and vectors generated by the trans - NIH KOMP initiative that aims to generate a public resource comprised of mouse embryonic stem (ES) cells containing a null mutation in every gene in the mouse genome.

The gene, known as gata5, acts in embryonic cells, which are primordial, unspecialized cells that form in the earliest stage of embryonic development and are genetically programmed to evolve into one of many specialized cell types, such as skeletal muscle cells, nerve cells, blood cells, skin cells, and liver cells.

April 2012 - New research: Illuminating embryonic stem cells Collaboration between two EU funded projects «Heroic» and «EuroSyStem», has provided new insights into embryonic stem cells The teams used next generation sequencing technology to examine two key properties of the cells that influence their identity and behaviour: gene expression and gene regulation.

BRD7, a novel PBAF - specific SWI / SNF subunit, is required for target gene activation and repression in embryonic stem cells.

Familial Dysautonomia (FD) Human Embryonic Stem Cell Derived PNS Neurons Reveal that Synaptic Vesicular and Neuronal Transport Genes Are Directly or Indirectly Affected by IKBKAP Downregulation.

My post-doctoral work on the identification of genes required for normal germ line development and fertility led to the discovery that the germ line is exquisitely sensitive to mutations in components of the mitotic spindle that have the potential to lead to aneuploidy — this sensitivity may also extend to embryonic and adult stem cells.