Sentences with phrase «facs cell sorting»

• NanoCellect Biomedical, a San Diego - based developer of microfluidic cell sorting technologies for cell - based assays, raised $ 10 million in series B funding.

Called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing, this new technique complements existing genomic tools, such as ChIP - seq (chromatin immunoprecipitation followed by sequencing), and offers some additional benefits.

At the University of Cologne, biophysicists in the lab of Professor Berenike Maier were now able to show how differential mechanical forces can lead to cell sorting in biofilms, thereby determining their architecture.

In a mix of bacteria that interact with each other to different degrees, the cells sorted themselves according to the mechanical forces they exerted among one another.

In the future, the question will be in how far this cell sorting strengthens the biofilm's resilience towards external stress.

Once the cells are in place, «the gel provides a natural - like environment in which the cells sort themselves out,» says University of Utah medicinal chemist Glenn Prestwich, who invented the formula.

Next, they used a technique called fluorescence - activated cell sorting to separate out the newly differentiated retinal ganglion cells from a mixture of different cells into a highly purified cell population for study.

The enlarged view shows a demonstration of on - chip cell sorting of a Euglena gracilis cell (time interval of each frame: 40?

Using a high - speed actuator as the driving source of pump, they succeeded in producing a flow with 16 microseconds for cell sorting.

Based on this technological advance, we are able to implement the principle of surface free energy - activated cell sorting.»

This simple protocol is based on automated fluorescent activated cell sorting (FACS) of OPCs.

Research at Nagoya University on cell sorting used a microfluidic chip to prevent sample infection.

A fluorescence - activated cell sorting (FACS) has been used for high - throughput cell sorting.

Human neural stem cells are derived via fluorescence - activated cell sorting (FACS) from donated fetal brain tissue.

An antibody that works well for Western blots on denaturing gels, for example, should not be presumed to work equally well in nondenaturing assays such as fluorescence - activated cell sorting.

«It's the same process as for single cell genomics, but for aggregates of symbiotic bacteria and archaea,» said DOE JGI Microscale Applications Group head Rex Malmstrom of the technique called BONCAT - FACS (BONCAT — Fluorescence - Activated Cell Sorting).

New research at Rockefeller University, published March 18 in Nature, reveals how cells sort out the loops meant to encode small RNAs, known as microRNAs, by tagging them with a chemical group.

Discovering that she could use a fluorescence - activated cell sorting machine to distinguish non-neuronal cells from neurons by making them glow in different colors was «a high point,» she says.

All of the following antibodies for both analytical and cell sorting cytometry were purchased from BD, except anti-CD27, which was purchased from eBioscience: anti — CD3 - PECy7 or PerCP, anti — CD20 - PECy7 or PerCP, anti — CD38 - PE, anti — CD27 - APC, and anti — CD19 - FITC.

↵ 3 The abbreviations used are: HUVEC, human umbilical vein endothelial cell; DiI - Ac - LDL, 1,1 ′ - dioctadecyl - 3,3,3 ′, 3 ′ - tetramethyl - indocarbocyanine acetylated low - density lipoprotein; FACS, fluorescence - activated cell sorting; FGFR, fibroblast growth factor receptor; Flk, fetal liver kinase; ICAM, intercellular adhesion molecule; mAb, monoclonal antibody; PE, phycoerythrin; TNF, tumor necrosis factor; VCAM, vascular cell adhesion molecule.

Our success in these areas is complemented by our substantial investment in cutting - edge facilities for nuclear magnetic resonance, protein crystallization, X-ray crystallography, transgenics, histochemistry, electron microscopy, confocal microscopy, and fluorescence activated cell sorting.

The emission wavelength used for cell sorting of DiI - Ac - LDL - labeled cells was 550 nm.

We also thank Robert Karaffa and Sommer Durham for providing essential support with cell sorting and Dr. Ruben Donis (CDC) for his generous gift of ferret anti-H5 serum.

Bethyl has applied B - cell sorting and recombinant DNA technology to our unparalleled on - site manufacturing process to deliver high quality recombinant rabbit monoclonal antibodies (RmAbs).

All live cell sorting and single cell sorting was performed on purified PBMCs using either a FACSVantage or ARIAII cell sorter system.

GFP - positive cells, indicative of reactivated latent HIV - 1, were isolated by fluorescence activated cell sorting (FACS).

300 μl of ice - cold 1 × binding buffer were then added to each sample and the samples were analyzed by fluorescence - activated cell sorting (FACS) within one hour for maximal signal.

Institutional needs for flow cytometry services were carefully evaluated and a decision was made to transition the operation of the South Campus Flow Cytometry and Cell Sorting Facility to an institutional core to provide the Flow Cytometry and Cellular Imaging Core with sufficient capacity needed to support the institution's investigators.

These include cell sorting and analytical flow cytometry to phenotype, quantify and isolate cells and confocal microscopy to capture high resolution images.p

The team has access to state - of - the - art laboratories and animal facilities with BSL2 and BSL3 containment, flow cytometry, cell sorting, histopathology and in vivo imaging (IVIS).

This Core provides flow cytometry and automated cell sorting services.

The Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cell sorting.

Committed to meet all of the flow cytometry needs for the Hubrecht Institute and the external research community, the Flow Cytometry Core facility offers state of the art instrumentation for routine flow cytometry and cell sorting.

The department has expertise and infrastructure to support bacterial and viral analysis, and the detailed evaluation of cellular and serological immune responses including flow cytometry and cell sorting, Luminex multiplex platforms, real time PCR, microarrays, comprehensive histological staining, immunohistochemistry and in - situ hybridisation.

Amani Said (Dahmann, MPG)-- «Mechanisms underlying cell sorting at the A-P compartment boundary in the Drosophila wing» (2007)

Advances in cell sorting and molecular analyses now enable researchers to comprehensively describe properties and functions of individual cells.

Katharina Landsberg (Dahmann, MPG)-- «The role of cell bond tension in directing cell sorting at the anteroposterior compartment boundary in wing imaginal dices of Drosophila melanogaster» (2010)

Human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), are known to be vulnerable to apoptosis upon various technical manipulation, such as single cell dissociation, freezing and thawing, etc., which hinder their use for clonal isolation in gene transfer, differentiation and FACS cell sorting.

Investigating the dynamics of cell sorting at compartment boundaries by real - time in vivo imaging

Increased cell bond tension governs cell sorting at the Drosophila anteroposterior compartment boundary.

• Flowmetric — Founded by CEO, Ren Capocasale, Flowmetric is a contract research organization focused on providing polychromatic flow cytometry and cell sorting services to bio-pharma and drug discovery companies.

By combining advanced approaches such as cell sorting and single cell analysis, researchers will gain a better understanding of the complex genetic landscape of disease.

Berkeley Lab's Len Pennacchio (left) and Axel Visel led the development of a new technique for identifying gene enhancers called SIF - seq, for site - specific integration fluorescence - activated cell sorting followed by sequencing.

Guidelines for sample preparation will be discussed prior to preparation, and well before arrival at the cell sorting lab.

The facility's staff will provide information for the preparation of samples for cell sorting.

His team applied, among others, techniques of confocal microscopy and cell sorting by flow cytometry which led to the discovery in human muscle biopsies that these myoendothelial cells are located adjacent to the walls of blood vessels.

To examine IL - 22 production specifically in ILCs, we isolated ILCs through cell sorting from Rag1 - / - mice.

The core facility offers assisted cell sorting services for characterizing, separating and purifying populations of particles as diverse as beads, micro-particles and cells.

A total of 143 colonies of the HS237 line cultured in the Cell - IQ equipment was used in fluorescence - activated cell sorting (FACS) analysis.

We acknowledge the OMRF FACS core and the OUHSC Flow Cytometry and Imaging Core for cell sorting and analysis.