Not exact matches
(I) Overexpressing either GBP1 or GBP2 reduces
FRE -
luciferase activity in the entire body.
(J) The gbp1, gbp2 ex67 mutant shows increased
FRE -
luciferase activity in the entire body.
In addition, overexpressing either GBP1 or GBP2 with TSC1 / TSC2 in the fat body reduced
FRE -
luciferase activity (Fig 7I).
The
FRE -
luciferase transgenic line [34] is a gift from Dr. Alexander W. Shingleton (Lake Forest College, IL).
FRE -
luciferase assays were performed as described previously [35] with a minor modification.
(I) Knocking down GBP1 and GBP2 increases
FRE -
luciferase activity in the entire body.
Next, we used the FoxO - Response Element (
FRE)-
luciferase construct to obtain a quantitative measure of FoxO activity [34 — 36].