FRET sensors face challenges of photobleaching, autofluorescence, and, in the case of exciting cyan - excitable donors, phototoxicity.
Another challenge to using
FRET sensors comes when employing optogenetic regulators to initiate the event being monitored.
Quantitative Imaging Approaches for Small - Molecule Measurements Using
FRET Sensors in Plants Plant Metabolism (pp. 55 - 64): Springer.
Flow Cytometry Enables Multiplexed Measurements of Genetically Encoded Intramolecular
FRET Sensors Suitable for Screening.
Dynamic imaging of cytosolic zinc in Arabidopsis roots combining
FRET sensors and RootChip technology.
Dynamic imaging of glucose flux impedance using
FRET sensors in wild - type Arabidopsis plants.
Carnegie researchers recently constructed genetically encoded
FRET sensors for a variety of important molecules such as glucose and glutamate.
Not exact matches
The biosensors are designed such that a change in activity is translated in a change in fluorescence intensity (intensity based biosensor), color (
FRET based biosensor) or location (translocation
sensor).
Many intracellular
sensors of events like calcium release have been made using fluorescent resonance energy transfer (
FRET) probes.