Sentences with phrase «fibroblasts for»

hESC lines I6 and H9 were maintained on Matrigel (BD Biosciences, Bedford, MA; http://www.bdbiosciences.com) coated dishes in medium (comprised of Dulbecco's Modified Eagle's Medium / Ham's F12 supplemented with 20 % knockout serum replacement (KSR), 2 mM non-essential amino acids, 4 mM L - glutamine, 0.1 mM β - mercaptoethanol, 50 µg / ml Penn - Strep, and 4 ng / ml of basic fibroblast growth factor) conditioned with mouse embryonic fibroblasts for 24 hours as previously described [9], [10].

Such a therapy would require a safe way to modify the fibroblasts for medical use.

SPRS - therapy is an autologous fibroblasts for cosmetic applications.

Not exact matches

Broiled salmon is also available at many Mexican restaurants for a healthy dose of omega - 3s (one of which research suggests may help protect against UV - induced skin damage) Eicosapentaenoic acid inhibits UV - induced MMP - 1 expression in human dermal fibroblasts.

The ingredient you are looking for is likely called Tissue Nutrient Solution or TNS ™, human collagen or human fibroblast.

The production of virus - free iPS cells, albeit from embryonic fibroblasts, addresses a critical safety concern for potential use of iPS cells in regenerative medicine.

Ding's team took cells called fibroblasts from the connective tissues of mouse fetuses and bathed them in a cocktail of the four polyarginine - tagged proteins for 12 hours, then they removed the reprogramming proteins for 36 hours, and repeated this cycle four times over.

The team also tested nanoparticle absorption for other kinds of human cells, including fibroblasts from donors of older ages and found significant differences.

The researchers — James Robl, a developmental biologist and his colleagues at the University of Massachusetts at Amherst, and Steve Stice at Advanced Cell Technology in Worcester, Massachusetts — inserted a marker gene fused with a gene for resistance to the chemical neomycin into a culture of connective tissue cells called fibroblasts.

«Fibroblast growth factor receptor inhibitors are new therapies being developed in clinical trials for patients whose cancer cells have genetic alterations in this family of genes,» says Roychowdhury, a member of the OSUCCC — James Translational Therapeutics Program.

For this study, Guttridge, first author David J. Wang, who developed many of the study's concepts, and their colleagues monitored NF - kB activity during tumor development using mouse embryonic fibroblasts and two mouse models.

For these clones, the donor cells were fibroblasts taken from fetuses.

To find out, John O'Neill, a biologist at the Medical Research Council's Laboratory of Molecular Biology in Cambridge, U.K., and his team studied skin cells known as fibroblasts, which are essential for wound healing.

Fibroblasts invade the void left by a scratch and lay the foundation for new skin to grow.

Fibroblasts play a key role in wound healing, laying the foundations for scar tissue to form.

The mycelia also appeared to act as scaffolding, attracting fibroblasts and anchoring them, ready for the next stage of wound - healing when collagen scar tissue forms.

Since it is known that 13 different members of the fibroblast growth factor, or Fgf, family act as signaling proteins between the two different tissues — dental mesenchyme and oral epithelium — during murine tooth development, Javed and colleagues looked for changes in Fgf ligands in the mice lacking Sp7.

For many years, tattoos were thought to work by staining fibroblast cells in the dermal layer of the skin.

The tissue, called the stroma, includes fat cells, or adipocytes, that provide padding; fibroblasts, which make the framework for tissue; pericytes in blood vessels, which are contractile cells that help regulate blood pressure; as well as myoepithelial cells comprising the outer layer of the ductal system through which milk flows.

«We also showed for the first time that AHR signaling in primary human orbital fibroblasts disrupts the pro-fibrotic Wnt / β - catenin pathway, which is important for scar cell formation,» noted Dr. Phipps.

Cells called cancer - associated fibroblasts arrange a normal meshwork of fibers into straight tracks, cell biologist Begum Erdogan of Vanderbilt University in Nashville and colleagues reported December 13 at the annual meeting of the American Society for Cell Biology.

WHEN I heard the sad news of Lonesome George «s death, my first thought was for his fibroblasts.

Their hypothesis was that the possible genes responsible for the pro-tumour role of the CAFs would be expressed at high levels only in these fibroblasts, and not in fibroblasts of the healthy tissue.

«Researchers discover a potential new therapeutic strategy for pancreatic cancer: Researches have have isolated a subpopulation of cancer - associated fibroblasts, which promote tumour growth.

For the new study, the team used a cell - reprogramming technique (similar to those used to reprogram skin cells into stem cells) to generate human DRG - type sensory neurons from ordinary skin cells called fibroblasts.

In these images of rat brains, differences in gene expression of genes for fibroblast growth factor 2 (FGF2, top set) and dopamine 2 receptor (D2, bottom sett) can be seen in rats of two breeds before (left) and after (right) cocaine exposure.

Rivolta and his colleagues knew that during embryonic development, a handful of proteins, including fibroblast growth factor (FGF) 3 and 10, are required for ears to form.

Expanding from their previous studies with mice, the researchers first established that under specific conditions, culturing human embryonic stem cells with fibroblast growth factor 2 (FGF2) leads to neural differentiation particular to the midbrain / hindbrain region — the location of the cerebellum — within three weeks, and the expression of markers for the cerebellar plate neuroepithelium — the part of the developing nervous system specific for the cerebellum — within five.

The key experiments included for replication were selected because they examine the induction of POSTN expression in the pulmonary stromal fibroblasts and test the role of POSTN in primary tumor formation and metastatic efficiency, which are relevant as the role of POSTN as a possible prognostic marker and target for anticancer therapies is explored (Xu et al., 2012; Nuzzo et al., 2014).

Individual cultures of 3T3 (a murine fibroblast line) and endothelioma cells subjected to identical treatment were used for negative and positive controls, respectively.

Cells were washed once with serum - free DMEM and then incubated in 10 % DMEM containing 10 μg / ml DiI - Ac - LDL for 4 h. Endothelioma cells and 3T3 fibroblast cells were used as a positive and negative reference, respectively.

Taken together, these results suggest that CSCs are essential for metastatic colonization and that CSCs induce stromal fibroblasts to secrete POSTN in the metastatic niche to support tumor cell outgrowth by augmenting the Wnt signaling pathway.

The scientists then looked at gene activation in insulin signaling pathways for iPSCs and fibroblasts with IR mutations, and for corresponding cells derived from people without those mutations.

A critical role for p38MAPK signalling pathway during reprogramming of human fibroblasts to iPSCs.

First, the MimEX ™ Cell Plating Procedure is used to maintain a uniform irradiated fibroblast monolayer for the rapid clonal expansion and efficient differentiation of tissue - specific adult epithelial stem cells.

Developed a novel - reprogramming paradigm for generating heart, brain and pancreatic cells directly from skin fibroblasts.

One of the many payoffs of the International HapMap Project was that it characterized genetic variation in fibroblast cell lines that could be ordered from Coriell for subsequent experiments.

Dr. Rubin said Curis had established a screening assay for SMN levels for a molecular library, first in fibroblasts from an SMA patient.

In future research supported by the National Institutes of Health, the researchers will study exactly how mucosal fibroblasts make immune cells more «infectable», which could ultimately lead to new targets for preventing HIV.

We have leveraged this knowledge to reprogram fibroblasts directly into cardiomyocyte - like cells for regenerative purposes.

Co-culture of bone marrow fibroblasts and endothelial cells on modified polycaprolactone substrates for enhanced potentials in bone tissue engineering.

The use of basic fibroblast growth factor (bFGF) for enhancement of bone ingrowth into pyrolized bovine bone.

Localization of all seven messenger RNAs for the actin - polymerization nucleator Arp2 / 3 complex in the protrusions of fibroblasts.

Also, intracellular milieu (ICM) is linked to extracellular milieu (ECM), what goes inside the cells also affects ECM because they are tied together and in communication; but also ECM is created by the ICM; for example, without fibroblast or stromal cells, for example, there wouldn't be much ECM or ECM scaffold formation in the first place; for they are key elements for ECM existing in the first place.

(6) Moreover, subcutaneous adipose of prematurely - aged transgenic mice exhibited high levels of staining for the senescence marker senescence - associated - β - galactosidase (SAβ - gal) and expressed high levels of several established markers of senescence, including p21, p19, interleukin - 6, (insulin - like growth factor binding protein - 2 (Igfbp2), and Pai - 1; primary BubR1H / H; INK - ATTAC mouse embryonic fibroblasts forced artificially into senescence by oncogenic Ras or serial passage exhibited a subpopulation that was both GFP + and stained positively SAβ - gal.

We prefer to culture our cells on mouse embryo fibroblasts (MEFs) but occasionally grow the cells feeder free for particular applications, such as nucleofection, viral transduction or karyotyping.

For example, we are reprogramming cardiac fibroblasts — the heart's connective tissue — directly into beating cardiac - muscle cells.

TEST CASE RESEARCHER Piroska Szabó, Associate Professor, Center for Epigenetics, Van Andel Research Institute ORGANISM Mouse METHOD RNA - seq In 2014, Szabó and colleagues reported how they'd used mouse embryonic fibroblasts to test whether the relatively new method of RNA sequencing could reveal known and novel imprinted genes.

The mRNA transcripts for the α3, α4, and α7 nAChR subunits were amplified from cerebellar cultures at 14 and 16 DIV and from renal fibroblasts.

The induced expression of these factors was accomplished through transducing the fibroblasts with, or making the fibroblasts uptake, a retrovirus vector that produced the DNA for these four proteins.