Sentences with phrase «flow cytometric»

Eight weeks postinjection, the engraftment was confirmed by flow cytometric staining of peripheral blood cells for human CD45 + / CD33 + cells, followed by randomization into study groups and treatment with a single bolus IV injection of IMGN632 or the control ADC.

(A, B) Mixed (1:1) CD11cDTR (CD45.1 +) and Ebi2 - / - or Ebi2 + / + (CD45.1 ʱCD45.2 +) BM chimeric mice were immunized with SRBCs and flow cytometric analysis of B cell responses performed five days later.

These may include but are not limited to vaccine specific and advanced multi-label flow cytometric analyses.

Since our flow cytometric data had shown that the HPCs poorly expressed MHC antigens [17], we wondered whether they stimulate allogenic MRL splenocytes (H2k).

A flow cytometric method to measure prokaryotic records in ice cores: an example from the West Antarctic Ice Sheet Divide drilling site Journal of Glaciology, in press, p. 1 - 19.

When absolute purity is not necessary, as is often the case with in vitro stimulation of T cells, magnetic cell separation can deliver highly enriched cells faster, with significant cost - savings, and without exposure to harsh separation protocols like flow cytometric sorting, or chemical gradients.

The homogenized fresh breast tumor samples have been represented for flow cytometric analysis using FACS calibur flow cytometer (Becton Dickinson, Sunnyvale, CA, USA) equipped with a compact air cooked low power 15 mwat argon ion laser beam (488nm), data analysis was conducted using DNA analysis program MODFIT (Topsham, USA).

Sensitive and viable identification of antigen - specific CD8 + T cells by a flow cytometric assay for degranulation.

Variation of flow cytometric DNA measurement in 1,485 primary breast carcinomas according to guidelines for DNA histogram interpretation.

Sections from all cases were subjected to stain with haematoxylin and eosin (H&E) for histopathological examination and immunohistochemical technique for Cyclin D1 in addition using flow cytometric technique to perform cell cycle analysis.

EdU incorporation and flow cytometric analysis were performed according to the manufacturer's instructions (Life Technologies, #C10634).

Annexin V staining and flow cytometric analysis were performed according to the manufacturer's instructions (ebioscience, # 88-8005-74).

To assess granzyme B production, CFSE - labeled cells were stimulated for 24 or 72 h, surface - stained as described above, permeabilized using a Cytofix / Cytoperm kit (BD Pharmingen), and then stained with flurochrome - labeled granzyme B Ab (GB11; Life Technologies) at 4 °C for 30 min prior to flow cytometric analyses.

Additionally, an aliquot of 4 × 106 splenocytes was stained for Foxp3 expression using methods and reagents, provided by the manufacturer (eBioscience), prior to flow cytometric analyses.

The facility supports a range of flow cytometric techniques and currently has 9 cytometers located at F2 - 33, F2 - 34, F2 - 35 and F2 - 36.

The instructors will focus on a broad spectrum of flow cytometric topics, discuss examples from various research applications, provide technical protocols for flow cytometric sample preparation, as well as data analysis, trouble shooting and experimental design.

The facility collaborates with various research projects using flow cytometric techniques to isolate and purify cells and chromosomes for structural and functional studies, sequencing, microarrays as well as fluorescence insitu hybridisation (FISH) experiment.

Subsequent single - cell flow cytometric assays linked this shorter G1 phase to the more rapid loading of minichromosome maintenance (MCM) helicase complexes when compared to differentiated cells with longer G1 phases (neural progenitor cells and retinal pigmented epithelial cells).

UNISI provides expertise in the measurement of cell signalling proteins, such as cytokines, chemokines and inflammatory biomarkers in multiple samples (including serum, plasma and tissue culture supernatants), using a multiplex suspension array system or a flow cytometric bead assay.

In this series of seminars, we will discuss the necessary tools to design, run, and analyze a multi-parameter flow cytometric experiment.

Not exact matches

Although conventional flow cytometry is limited to measuring particles down to approximately 300 nm — 500 nm, a relatively new flow - cytometric method — called «imaging flow cytometry» — allows for the analysis of EVs smaller than 300 nm.

Technical support is also provided for analyses of flow and imaging cytometry data for publication, presentation, and inclusion in grant applications, management of cytometric data (storage, archiving, and retrieval), and management of a site license for low - cost analysis software.