Not exact matches
The 2 alleles differ by a single nucleotide, so restriction digestion of the amplified product
followed by gel
electrophoresis effectively differentiates the 2 alleles.
PCR - based assays identify clonality on the basis of over-representation of amplified V — D — J (or incomplete D — J) products
following their separation using gel or capillary
electrophoresis.
Using Surveyor nuclease
followed by gel
electrophoresis, these mismatches are indicated by the presence of DNA cleavage products.
Nondenaturing 2 - dimensional gradient gel
electrophoresis followed by apoA - I immunoblotting was performed on plasma from the patient and 17 control participants.20