Sentences with phrase «following electroporation»

Following electroporation into R26 BirA cells, a cell line that expresses bacterial BirA ligase from the ROSA26 locus, hygromycin resistant clones (200 µg / ml) were expanded for Southern analysis to identify correct targeting events.

Not exact matches

INO - 3112 is injected into a muscle followed by electroporation, a technique that uses controlled, millisecond electrical pulses that make it easier for the DNA plasmids to enter muscle cells.

HV cells constitutively expressing the LacZ gene were generated by electroporation with a vector containing a CAG driven β - Geo cDNA followed by selection in G418 (150 µg / ml) for 2 wk.

HV cells overexpressing Nanog were generated by electroporation with a vector containing the Nanog cDNA under the control of a CAG promoter and upstream of IRES Puro cassette followed by selection in puromycin (2 µg / ml) for 2 wk.

1 - 4 days following DNA injection and electroporation, embryonic brains are dissected, sliced, placed on a filter in culture medium and live imaged over night (Baffet et al., Methods Cell Biol., 2015).