Sentences with phrase «for cell culture supernatant»

Not exact matches

Vaccinia virus (Western Reserve) recombinant for the codon - optimised gp120 gene from HIV - 1 (Bx08) expressing gp120 protein in (VERO) cell culture supernatant.

In this study, we have developed a new sensitive and specific one step rRT - PCR for detection of ZIKV in serum and cell culture supernatants using a Taqman probe containing locked nucleotides, in the NS5 region of ZIKV genome using sequences of ZIKV strains circulating in Africa and Asia.

Cell debris was removed from culture supernatant by centrifugation at 12,500 g for 45 min at 4 °C, and the virus particles were concentrated by ultracentrifugation at 45,000 r.p.m in a BECKMAN 70Ti rotor for 3 hrs.

Cells were cultured under normal culture conditions for 24 h and the supernatants were subjected to PGE2 ELISA.

The cell culture supernatant was collected and concentrated if necessary, and then 100 μL aliquots were used for detection with ELISA kit (PBL Biomedical Laboratories) following the protocol of the manufacturer.