Sentences with phrase «for immunoblot»
Similar protocols were used for immunoblot analysis of cell protein extracts from MC3T3 - E1 (mouse preosteoblasts), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblasts with osteogenic potential).
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As further evidence of BMP signaling activation, proteins isolated from zebrafish embryos were examined by immunoblotting for phosphorylated Smad1 / 5.
Lysates were immunoblotted for protein levels of (A) phosphorylated ERK (pERK), total ERK (tERK), and β - actin or (B) phosphorylated IκBα (p - IκBα), total IκBα (IκBα), and β - actin.
Suitable for ELISA, immunoblotting, RIPA and IF.
Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).
Immunoblots were interpreted by criteria of the Centers for Disease Control and the Association of State and Territorial Public Health Laboratory Directors [31].
Cell lysates were harvested and subjected to immunoblot analysis for MEK1, MEK2, MKK3, MKK6, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 (t - ERK1 / 2), phosphorylated p38 (p - p38), total p38 (t - p38) and actin.
Brain, eyes, and optic nerves were taken from transgenic and wild - type mice of 3 to 12 months of age and processed for immunohistochemistry, qPCR, or western immunoblotting.
A band consistent with the entire ectodomain was observed by immunoblotting for the majority of the proteins and their expression levels were quantified.
Samples were prepared for cell cycle analysis and immunoblotting.
The indicated cells were untreated or treated with doxycycline for 4 days followed by immunoblotting and imaging analyses.