Sentences with phrase «for immunoblot analysis»
Similar protocols were used for immunoblot analysis of cell protein extracts from MC3T3 - E1 (mouse preosteoblasts), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblasts with osteogenic potential).
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Cell lysates were harvested and subjected to immunoblot analysis for MEK1, MEK2, MKK3, MKK6, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 (t - ERK1 / 2), phosphorylated p38 (p - p38), total p38 (t - p38) and actin.
Samples were prepared for cell cycle analysis and immunoblotting.
The indicated cells were untreated or treated with doxycycline for 4 days followed by immunoblotting and imaging analyses.