All JAK1 and JAK2 mutants were generated using QuickChange XL II site - directed mutagenesis kit (Stratagene) and
subcloned into the pMX - GFP (
for JAK1) or pMEGIX - GFP (
for JAK2) biscistronic retroviral vector upstream of the IRES as previously described.17 — 19 The mutated JAK1 and JAK2 constructs were verified by sequencing the full - length JAK1 and JAK2 using DYEnamic ET Dye Terminator Kit (Amersham Biosciences).
Tags
for this Online Resume: Lentiviral Retroviral shRNA RNA knockdown screens, Flow Cytometry, RTPCR, Immunopreciptations, Reporter assays, Cell based Assays, ELISA, Migration, Stable and Transient Transfections, Genomic and Proteomic Studies, Cloning, Transient siRNA knockdowns, Phenotyping Mammalian cell lines, Gene Overexpression,
Subcloning to expression Vectors, Construct Stable Inducible Cell lines, Protein Kinase Assay, Pulse Chase Experiments, Transfection, Transduction, Transformation, Polyoma virus DNA tumor virus replication, Research Scientist, Cell Biology Protein Biochemistry Genetics Molecular Bioliogy Oncology Virology, FACS