Sentences with phrase «gfp expressing cells»

The GFP expressing cells were > 70 % CD45 by day 26 (A).

We then tracked the expression of CD45 and class I and class II molecules on HPC - derived cells in long - term chimeric Rag2γc − / − mice by gating for the GFP expressing cells.

Not exact matches

Single - cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter.

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells.

5 - HT3A - driven GFP was not expressed in taste receptor cells.

In mice expressing GFP under the control of the 5 - HT3A promoter, a subset of cells in the geniculate ganglion and nerve fibers in taste buds are GFP - positive.

Kaplan - Meier analysis demonstrated that most of the mice injected with cells expressing wild - type PREX2 or GFP exhibited tumor - free survival for more than ten weeks.

We observed similar robust increases of intracellular calcium concentrations in response to KCl and ATP in both GFP - expressing (n = 28) and GFP - nonexpressing cells (n = 12), whereas 5 - HT elicited an increase in intracellular calcium only in GFP - expressing cells (KCl: t (40) = 0.98, p = 0.33; ATP t (38) = 1.04, p = 0.30; 5 - HT: t (38) = 5.24, p < 0.0001 Student's t tests; Fig. 4A, B, F).

Control studies were performed using cells expressing either wild - type PREX2 or green fluorescent protein (GFP).

B, Histograms of GFP and P2X3 normalized immunoreactivity of individual cells showing that whereas the P2X3 staining shows a unimodal normal distribution (mean 0.58), the GFP population is bimodal suggestive of high - expressing (mean = 0.69) and low - or nonexpressing populations (mean 0.12).

E, Histograms of GFP and FG normalized fluorescence intensity of individual cells showing bimodal distributions suggestive of a high - expressing population (mean GFP: 0.59, FG: 0.62) and a nonexpressing population (mean GFP: 0.08, FG: 0.08).

Time - lapse movie of zebrafish lens epithelial cells expressing GFP - histones and mCherry - zGem as they progress through the cell cycle.

The GFP - expressing nerve fibers often were closely associated with serotonergic type III cells (Fig. 3A — D), suggesting the possibility of serotonergic transmission between serotonergic type III taste cells and 5 - HT3AGFP - expressing nerve fibers.

They utilized the mouse line in which GFP is expressed in cells, but have an active TH promoter.

MDCK cells permanently transfected for expression of LifeAct - GFP (green) were grown as polarized monolayers and infected with Salmonella expressing mCherry (red).

We have introduced chimeric genes which express GFP in a variety of organelles within the plant cell in order to study the dynamics of cell organization.

GFPs enable researchers to watch live cells at work: If they're interested in a certain protein that can be expressed by an organism, they can fuse the GFP gene with the protein - encoding gene.

Conditioned medium from both GBP1 and GBP2 expressing cells induced lower levels of ILP2 and ILP5 accumulation than GFP conditioned medium.

The researchers next assessed the function of the NK cells to inhibit HIV replication using the CEM - GFP T - cell line which when infected with HIV - 1 expresses GFP.

In the original publication they see GFP expression in about 20 % of their Cas9 - expressing cells.

GFP expression was not detected in the Repo - expressing BBB glial cells (arrowheads in H and I).

GFP was present in all four SIFa - expressing cells located in the anteromedial region of the brain (arrows in F).

I thought I could see GFP expression with a fluorescence microscope, but the GFP expression was so low (even in the transduced wild - type A549 cells that lack Cas9 and thus should have expressed GFP) that I couldn't get a good image.

Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types to do more than just label cells.

When tested in retinal electroporation studies, DsRed reporter expression was induced in ~ 76 % of GFP + cells, and 100 % of DsRed + cells also expressed GFP.

Neighboring GFP - labeled cells express NPF or SIFamide (S1 Fig).

(A, B) Neurite length of neurons in E14 rat VM cultures transfected with control siRNA, Smad4 siRNA or Smad1 siRNA or Smad5 siRNA and a GFP - expressing plasmid before being cultured with or without 10 ng / ml GDF5 for 72 h (*** P < 0.001 compared with control; ANOVA with post-hoc Tukey's test; 40 cells for each group per experiment; number of repetitions (n) = 3 experiments).

(A) Neurite length and (B) neurite branching of SH - SY5Y cells transfected with control siRNA or Smad1 siRNA or Smad5 siRNA and a GFP - expressing plasmid together with a control or miR - 181a antagomir for 72 h (* P < 0.05, *** P < 0.001 compared with control)(# P < 0.05, ## P < 0.01, ### P < 0.001 ANOVA with post-hoc Tukey's test; 40 cells for each group per experiment; n = 3 experiments).

Several cells express the GFP, fluorescing in green, when excited with blue light.

In this medium, the HOXB4 gene is activated and stoichiometrically expressed at a 1 ∶ 1 ratio with GFP, so that the cells turn green as they become hematopoietic.

(A) In wild - type (wt; N2), UNC - 9:: GFP expressed in B motor neurons (Pacr - 5:: unc - 9:: gfp) visualizes puncta in the ventral nerve cord localized near B cell bodiGFP expressed in B motor neurons (Pacr - 5:: unc - 9:: gfp) visualizes puncta in the ventral nerve cord localized near B cell bodigfp) visualizes puncta in the ventral nerve cord localized near B cell bodies.

Mixed chimerism was monitored by measuring by flow cytometry GFP - expressing CD45 + cells over 100 days.

INX - 4:: GFP does not appear to be expressed in neurons implicated in locomotion, but is expressed in a number of ciliated neurons, pharyngeal m1 muscle cells, and the RIP neurons that form gap junctions with pharyngeal I1 neurons (Figure 5E).

(B) UNC - 9:: GFP is expressed more uniformly in the ventral nerve cord in unc - 7 (e5) animals, and clusters of puncta near B cell bodies are not discernible.

(E) UNC - 7L expressed in B motor neurons (Pacr - 5:: unc - 7L [M121L]-RRB- in unc - 9 daf - 6 unc - 7 does not localize to puncta but remains associated with cell bodies (red); co-expressed UNC - 9:: GFP (Pacr - 5:: unc - 9:: gfp; green) is distributed throughout the motor neuron processGFP (Pacr - 5:: unc - 9:: gfp; green) is distributed throughout the motor neuron processgfp; green) is distributed throughout the motor neuron processes.

(D) In unc - 9 daf - 6 unc - 7 animals, Pacr - 5:: unc - 9:: gfp is expressed more diffusely in cell bodies (few bright puncta).

UNC - 9:: GFP was widely expressed in the ventral nerve cord as puncta, but UNC - 7L was principally associated with cell bodies, and no co-localization was observed (Figure 10E).