Some GFP nanobodies stain poorly when tagged with a Spaghetti Monster because they recognize this GFP scaffold itself.
Not exact matches
Nanobodies binding
GFP were designed in 2009.
Subsequent work showed that
nanobodies could be fused to other proteins, and these fusions retained the ability to bind
GFP.
Once Tang and Cepko found a description of
GFP - binding
nanobodies, the project really took off.