Sentences with phrase «hff culture medium»

The objective of the Islamic slaughter is to ensure maximal blood drainage from the animal as Muslim belief blood is a good culture medium for microorganisms to flourish.

«When someone has a problem — whether they need to find a particular cell line or need to understand the formulation of a particular type of culture medium — they're coming to you and they're in a crisis.

Adding ascorbic acid to culture medium could help overcome the main roadblock in reprogramming human, mouse cells into iPS cells

When the scientists succeeded in collecting the queen, part of the colony was transported to the laboratory, and all the bacteria found on the insects» surface and inside their bodies were isolated, characterized, and placed in culture medium.

To find out, they put droplets of culture medium that cancer cells had grown in on one side of petri dishes.

When the Stanford team measured pyrophosphate levels in cultured cells derived from ank and normal mice, they found that the chemical accumulated in cells from the ank mice but decreased in the culture medium.

The cells are grown in the incubator in different concentrations in a culture medium.

When glucose is added to the culture medium for cows and sheep embryos, a greater number of males survive.

Draths and Frost were able to complete the final stage, in which muconic acid is converted to adipic acid by hydrogen gas and a platinum catalyst, without having to separate the muconic acid from the crude culture medium.

Hang and coworkers exposed the human cells by first extracting the compounds from the paper with a culture medium then using the medium to culture the human cells for 24 hours.

Although electrolyte leakage is still undesired, its danger is minimized by the use of either the normal saline solution pumped into the body in most IV treatments or a cell - culture medium that contains amino acids, sugars, and vitamins in addition to sodium ions and thus mimics the fluid that surrounds human cells.

Previous work by the researchers developed plasma - activated cell culture medium as a form of chemotherapy, but selected Ringer's solution in the present work because of its simpler composition and likelihood of forming less complex reaction products.

Researchers in China have engineered bendable batteries that can run on body - inspired liquids such as normal IV saline solution and cell - culture medium.

Following cultivation of Arabidopsis seedlings on a culture medium containing a high concentration of cesium carbonate, Cesium Green was applied to the seedlings, and the resulting green fluorescence observed was used to confirm the presence of cesium within the plants» cells.

The bacteria would need either to synthesize the new genetic letters themselves or to import them from the surrounding culture medium.

The resulting organoids could either be banked for future use or immediately fragmented and seeded into the «epithelial» channels of multiple Organ Chips where they were further matured by flowing specialized culture medium and applying mechanical stimulation to the channels to mimic physiological peristalsis - like motions.

To confirm that the phosphate was biological in origin, the German researchers added human faeces to a culture medium and then incubated the samples under anaerobic conditions at a temperature of 36 °C.

One possible pilot project would create human cells that can tolerate a simpler and less costly culture medium by surviving without the typical amino acids and vitamins.

«Spinal fluid is a great culture medium — one of the best,» he says.

The group reported growing multiple parthenogenetic embryonic stem cell lines by incubating eggs in a warm, low - oxygen culture medium.

When they put human macrophages in the lower compartment filled with cell culture medium, the cells rose toward the membrane.

Maures et al. (p. 541, published online 29 November) explored why the presence of abundant mating - competent males causes a decrease in the life span of nematodes of the opposite sex and found that a secreted substance, possibly a pheromone, reproduced the effect of the males when transferred in the culture medium.

The kit adopts a novel purification method, using magnetic beads and phosphatidylserine - binding protein to isolate exosomes and other EVs from cell - culture medium and body fluids by a normal microcentrifuge.

But if the culture medium contains an anti-inflammatory molecule, this response is blocked.»

The addition of a small amount of venom to the culture medium in the absence of the extract of sarcoma 180 elicited the growth of the same dense halo of nerve fibers around an isolated sensory or sympathetic ganglion.

When NGF is withdrawn from the culture medium, the cells retract their fibers, lose their other neuronal properties and resume the uncontrolled proliferation characteristic of neoplastic cells.

Culture medium was changed every 5 d.

EGCG or DAC was added, in new culture medium, to the cells on days 1, 3, and 5.

Signal sequence of env replaced by tpa to direct secreation of rgp105 into culture medium

To address this question, we plated these cells on microwells, transduced the cells one day after and then switched to a neuronal culture medium as low oxygen.

The culture medium was collected after 24 hr of conditioning and albumin content was analyzed with the Albuwell kit (Exocell) according to the manufacturer's protocol.

Accumulation of lipids to their maximum level occurred when cells began to synthesize amino acids in the presence of excess glucose - the major carbon source in the culture medium.

Stable transfectants were selected in culture medium containing G418 (1mg / ml).

None of these toxic effects were observed when the same cells were treated with culture medium from non-senescent astrocytes that had not been subjected to paraquat.7

After 2 — 3 weeks, propagated cells were dissociated with 0.25 % trypsin, resuspended with culture medium, and cultured for a further 2 — 12 weeks.

One - half of the culture medium was replaced with a fresh medium every 3 — 4 days for the 16 DIV.

Confirmation that the visualized spirochete was B. burgdorferi was done by PCR on a sample of the culture medium, as reported elsewhere [25].

Thirdly, HFF cells are more tolerant to certain reagents added to the culture medium.

To measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red.

degraded after the addition of auxin to the culture medium.

By activating a gene called Ras in cells bathed in a very specific culture medium, scientists were able to cause embryonic stem cells — which originate from the inner cell mass of the blastocyst — to become more like the trophoblastic stem cells that give rise to the placenta from the outer portion of the blastocyst.

For example, Masato Kanemaki's lab has developed a CRISPR / Cas - based system for tagging endogenous proteins with an auxin - inducible degron (AID) tag to generate proteins which can be rapidly and reversibly degraded after the addition of auxin to the culture medium.

Forty - eight hours after transfection, the media were centrifuged and the supernatant was diluted with culture medium to 100 %, 50 % and 25 % the original concentrations.

It is also worthy to mention that human ES cells and iPS cells could be maintained on the HFF cells with the conventional human ES culture medium (Li et al, unpublished data).

Subsequently, the culture medium was returned to the HFF medium without LIF.

For monolayer differentiation, confluent iPS cells were cultured in the HFF culture medium without any growth factors.

When conditions improve (or when the scientist restores nitrogen to the culture medium and provides light and water), the diploid zygote undergoes meiosis and releases four haploid cells that resume the vegetative life cycle.

Peptides were added to the culture medium, and fluorescence changes from GCaMP6s were measured by live imaging.

The colonies were cultured in suspension in Petri dishes with ES cell medium [14], and the culture medium was changed every 3 days.

EBs were cultured in 6 - well ultra-low attachment plates (Corning, Lowell, MA), and the culture medium was changed every 3 days.