Sentences with phrase «hts cell»

The intermediate reticular zone is a key extraraphé component of the medullary serotonin (5 - hydroxytryptamine [5 - HT]-RRB- system that contains 5 - HT cell bodies.

These findings raise the question as to how reduced 5 - HT and TPH2 levels are related to the increased 5 - HT cell density, 11 morphologic 5 - HT neuronal immaturity, 11 reduced 5 - HT transporter binding relative to 5 - HT cell number, 11 and altered 5 - HT receptor binding9 - 11 in the SIDS cases.

Second, in components of the medullary 5 - HT system that contain 5 - HT cell bodies (ie, PGCL, gigantocellularis, and intermediate reticular zone), there was a significant age × diagnosis interaction with decreased receptor binding with increasing age in SIDS cases but no change in controls (Figure 2A).

Not exact matches

Serotonin (5 - hydroxytryptamine, or 5 - HT) is a monoamine neurotransmitter synthesised in serotonergic neurons in the central nervous system and enterochromaffin cells in the gastrointestinal tract.

Using a method known as high throughput sequencing (HTS), the team was able to determine how many distinct malignant cells were present within a sample of healthy cells.

This particular type of cell - based HTS assay is used by many of the fast - track biotechs and pharmaceutical companies, and they take good care of their people.

C, D, Ca2 + signals from GFP + geniculate ganglion cells in response to 10 μm ATP, 10 μm ATP + 1 μm ODS, 10 μm 5 - HT, 10 μm 5 - HT + 1 μm ODS, and 55 mm KCl (C) or 10 μm ATP, 10 μm CPG, 10 μm CPG + 1 μm ODS, and 55 mm KCl (D).

In the present study, we have investigated the role of 5 - HT in the communication between taste receptor cells and afferent nerve fibers.

Therefore, 5 - HT signaling to afferent nerve fibers could participate in the transmission of sour or some components of salty taste by type III cells.

For acids, the taste of which is mediated by type III cells, one possibility is that ATP is coreleased from vesicles along with 5 - HT.

C, HT - 29, KYSE150, and PC3 cells were treated with 5 or 20 μM of EGCG for 6 days under conditions described in «Materials and Methods.»

We observed similar robust increases of intracellular calcium concentrations in response to KCl and ATP in both GFP - expressing (n = 28) and GFP - nonexpressing cells (n = 12), whereas 5 - HT elicited an increase in intracellular calcium only in GFP - expressing cells (KCl: t (40) = 0.98, p = 0.33; ATP t (38) = 1.04, p = 0.30; 5 - HT: t (38) = 5.24, p < 0.0001 Student's t tests; Fig. 4A, B, F).

The GFP - labeled fibers are closely associated with 5 - HT labeled type III cells (white arrows) in all taste fields.

Our physiological analysis established that functional 5 - HT3 receptors are present only in these GFP - labeled ganglion cells and that 5 - HT3 is required for ganglion cell responses to 5 - HT.

In taste buds, 5 - HT is synthesized, stored, and ultimately released by type III taste cells that respond to acidification (Huang et al., 2009, 2011) or high levels of NaCl (Oka et al., 2013).

F, Average responses to KCl, ATP, and 5 - HT in GFP + and GFP − ganglion cells.

Rather, sour tastants evoke release of serotonin (5 - hydroxytryptamine; 5 - HT) directly from type III cells (Huang et al., 2005, 2009).

The ability of the specific 5 - HT3 antagonist to block Ca2 + responses indicates that 5 - HT3 is the only 5 - HT receptor capable of generating a Ca2 + signal in the ganglion cells.

Arrows highlight sites of convergence between 5 - HT - expressing type III cells and 5 - HT3AGFP - expressing nerve fibers.

Type III taste cells release 5 - HT directly in response to acidic (sour) stimuli and indirectly in response to bitter and sweet tasting stimuli.

For the time course study, cells were treated with 20 μM of EGCG for 12, 24, 48, 72, or 144 h. Human colon cancer cell line HT - 29 and prostate cancer cell line PC3 were obtained from American Type Culture Collection (Manassas, VA), and were grown in McCoy's 5A and RPMI 1640 containing 10 % fetal bovine serum, respectively.

E, ATP (circles) and 5 - HT (triangles) dose — response curves for 5 - HT3AGFP - expressing geniculate ganglion cells.

The 5 - HT released by type III cells could also activate 5 - HT3 receptors on afferent nerve fibers.

5 - HT released by type III taste cells activates adjacent type II taste cells that express the 5 - HT1A receptor, resulting in inhibition of type II cells (Herness and Chen, 2000; Huang et al., 2005; Jaber et al., 2014).

The serotonergic type III taste cells appear to contact the 5 - HT3AGFP - expressing taste nerve fibers, which then are positioned appropriately to respond to a 5 - HT signal.

These findings suggest that 5 - HT released from type III taste cells might activate gustatory sensory terminals in taste buds.

Herein, we test whether 5 - HT released by taste cells plays a role in the transmission of taste information by activation of 5 - HT3 receptors on afferent nerve fibers.

Phenotypic stability and unlimited self - renewal capacity of cell derived from pluripotent stem cells are essential properties for cell assay development for High Throughput Screening (HTS) strategies.

We will extend our HTS tests using different cell assays and extended chemical components libraries.

The main perspective is to use Induced Pluripotent Stem (iPS) cells as a biological model for the next HTS screenings.

Then, the first objective of the HTS platform is to develop a robust cell based assay in microplates (96 - 384 - wells) with a specific read out.

The HTS at I - Stem focuses on relevant biological models of genetic diseases: the human stem cells and their progenies.

By intracellular and whole cell recording in rat brain slices, it was found that bath - applied serotonin (5 - HT) produces an increase in the frequency and amplitude of spontaneous excitatory

STAT6 specific shRNA inhibits proliferation and induces apoptosis in colon cancer HT - 29 cells.

HT - 1080 fibrosarcoma cells were supplied by ATCC and maintained in culture as described by the provider.

In addition, knockdown of STAT6 by short hairpin RNAs results in apoptosis of human colon cancer HT - 29 cells (Zhang et al., 2005).

The gelatinolytic assays were carried out in two different ways to obtain complementary information: firstly, cells were treated or not with the test compound and samples from these were submitted to gelatinase zymography to detect the effects of the kahweol treatment on the expression of gelatinase activities; secondly, in some experiments, samples from control, untreated HT - 1080 fibrosarcoma cells were submitted to zymography and, after electrophoresis, different concentrations of kahweol were added to the substrate buffer to determine the potential direct effect of kahweol on gelatinase activity.

Acquired tumor cell resistance to sunitinib causes resistance in a HT - 29 human colon cancer xenograft mouse model without affecting sunitinib biodistribution or the tumor microvasculature

Instead of growing cancer cells on plastic, they inserted a multi-layered omental tissue culture model into each well of a 384 - well or 1536 - well HTS platform.

The numbers of ovarian cancer cells that adhered to and invaded the HTS model were counted, and the inhibitory potential of each compound evaluated.

A patent application has been filed and testing is underway on the protein — called HT - TALENs (short for HIV - targeted transcription activator - like effector nucleases)-- which uses a newly developed gene - editing technique to rid the body's cells of the immunodeficiency virus before it has a chance to multiply and possibly develop into AIDS.

This innovative platform provides ChIP services followed by library preparation in a cost effective high - throughput (HT) format from cell lines and tissues to assess the chromatin - enrichment of well - established histone marks or your protein of interest.

Antirotaviral activity of milk proteins: lactoferrin prevents rotavirus infection in the enterocyte - like cell line HT - 29, Med Microbiol Immunol 186, 83 - 91.

So far, we've reviewed Cell Mass, True Mass, and Axis HT.

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A recent Pew survey (HT: The Panic Virus) found that «across the board, people who use the internet and other digital devices — such as cell phones, tablet computers, and e-book readers — are more frequent readers than non-users.»

Conducted MTS and HTS functional screens (mGLUR2, mGLUR4, mGLUR5 and mGLUR7) and performed Dissociation and Association Kinetic Assay using FLIPR calcium mobility assay on cells transfected with metabotropic glutamate receptor.

Proteomes covers the multi-disciplinary topics of structural and functional biology, protein chemistry, cell biology, methodology used for protein analysis, including mass spectrometry, protein arrays, bioinformatics, HTS assays, etc..

Tags for this Online Resume: High - Throughput (HTS) and High - Content Screening (HCS), Research Scientist, Laser Scanning Confocal Microscopy and Spectrophotometry Usage and Analysis, Murine manipulation, • ELISA • Western Blot, Flow Cytometry, Microbiology and Immunology, cell - based assays, molecular biology techniques