This thesis primarily investigates the effects of mechanical cyclic stretching, a 5 % low oxygen atmosphere and the Rho - kinase inhibitor, Y - 27632, on protein responses in chondrocytic
human chondrosarcoma (HCS - 2 / 8) cells.
Human chondrosarcoma cells were stretched to 8 % strain at a frequency of 1 Hz.
In this study, we investigated the phosphorylation events in
human chondrosarcoma cells during cellular stretching.
Rho - kinase inhibitor Y - 27632 and hypoxia synergistically enhance chondrocytic phenotype and change the S100 protein profile in
human chondrosarcoma cells
Human chondrosarcoma HCS - 2 / 8 cells, which are a type of benign bone cancer cells, can be used to investigate how optimization of culture conditions could improve the synthesis of cartilage - specific molecules.»
The results showed that, when applied, a long - term inhibition of Rho - kinase signaling increased the expressions of chondrocyte - specific genes and differentiation markers in
human chondrosarcoma 2/8 cells.