Sentences with phrase «hybridization buffer»

A 10 µl of probe hybridization mix was prepared by mixing 100 ng XMRV - SO, 100 ng CEP8 - SA, 1000 ng sonicated human placental DNA, 250 ng human Cot - 1 DNA, and 7 µl LSI / WCP hybridization buffer (Abbott Molecular, Inc.), and was added to each slide over the cell specimen.

A 10 µl of probe hybridization mix was made by mixing 100 ng XMRV - SO, 100 ng CEP8 - SA, 1000 ng sonicated human placental DNA, 250 ng human Cot - 1 DNA, and 7 µl LSI / WCP hybridization buffer, and was dropped to each slide over the tissue section.

The riboprobes were diluted in hybridization buffer (50 % formamide, 10 mM Tris - HCl, 200 µg / ml tRNA, 1 × Denhardt's solution, 10 % dextran sulfate, 600 mM NaCl, 0.25 % SDS, 1 mM EDTA at pH 7.6), heat - denatured at 85 °C for 10 min, and then added to each slide.

This panel combines new generations of FISH technologies, pairing oligonucleotide - based SureFISH technology with a ready - to - use formulation of formamide - free IQ Hybridization Buffer, resulting in high signal - to - noise ratios with less than 4 hours of turnaround time.

Not exact matches

The hybridization was performed at 50 °C overnight in 50 % formamide buffer [0.5 ng / mL probe, 1 mg / mL tRNA, 0.1 mg / mL poly (A), 30 mM DTT, 0.3 M NaCl, 10 mM Tris - HCl, 1 mM EDTA, 10 % dextran sulfate, and 1 × Denhardt's solution].