Sentences with phrase «i immunoblotting»

Protein immunoblot and histochemical analysis with antiserum to type I NO synthase suggest that the formation of NO in pancreatic B cells is catalyzed by an NADPH -(reduced form of nicotinamide adenine dinucleotide phosphate), Ca2 + / calmodulin - dependent type I NO synthase of about 150 kilodaltons.

In this webinar we will explore the issues of reproducibility in immunoblotting in more depth and discuss how to obtain higher quality data, with a focus on those key factors necessary to create consistent, quantifiable results.

Learn how different detection technologies such as chemiluminescence and fluorescence can affect the quality of immunoblotting data

As further evidence of BMP signaling activation, proteins isolated from zebrafish embryos were examined by immunoblotting for phosphorylated Smad1 / 5.

Immunoblot analysis.

Similar protocols were used for immunoblot analysis of cell protein extracts from MC3T3 - E1 (mouse preosteoblasts), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblasts with osteogenic potential).

Lysates were immunoblotted for protein levels of (A) phosphorylated ERK (pERK), total ERK (tERK), and β - actin or (B) phosphorylated IκBα (p - IκBα), total IκBα (IκBα), and β - actin.

RT activity, Immunoblot reactive, IFA

Suitable for ELISA, immunoblotting, RIPA and IF.

Normalized protein lysates were subjected to SDS - PAGE, transferred to nitrocellulose, and immunoblotted with primary Abs from Cell Signaling Technology (Danvers, MA) at concentrations recommended by the manufacturer.

Quantification of rapid Myosin regulatory light chain phosphorylation using high - throughput in - cell Western assays: comparison to Western immunoblots.

Antibodies to env, gag and pol are seen in an immunoblot

HIV - 1 p55 / p24 by immunoblot (weak reactivity).

Reacts with p66 / p51 in immunoblots and inhibits the reaction of RT enzyme in a Biological assay.

Western Blotting is an analytical Immunoblotting Technique to detect specific proteins in a cell extract or tissue homogenate.

Immunoblots showed almost complete inhibition of mTOR expression (as shown in Fig. 4B) that resulted in decreased cell proliferation as assessed by MTS assay (A).

HuH - 7 cell extracts (lane 3) or FLAG - Sp1 - containing cell extracts (lane 4; 1.5 mg each) were immunoprecipitated with anti-FLAG antibody — conjugated beads and the immunoprecipitates were analyzed by SDS - PAGE followed by immunoblotting with anti-FLAG antibody or anti-DDX3 antibody.

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

Equal amounts of protein were solubilized in 2.5 x SDS - sample buffer, separated on 12 % SDS - polyacrylamide gels, transferred to Immobulin P and immunoblotted with the antibodies indicated in the Materials section.

Total cell lysates of HuH - 7 cells were incubated with GST or GST - Sp1 fusion protein — prebound glutathione - Sepharose 4B beads and the bound fractions were subjected to immunoblotting with antibody against DDX3 (top).

Immunoblots showed decreased levels of p - mTOR and p - p70S6K after treatment with increasing concentrations of rapamycin.

Immunoblots showed strong expression of HA tag in infected ALCL cells but not in control (uninfected or infected with adeno - β - Gal) cells (A).

Protein was abolished as shown by Northern blot and immunoblot analysis.

Immunoblots showed a concentration - dependent decrease in the antiapoptotic proteins BCL - 2, BCL - XL, MCL - 1, and c - FLIP (long and short) with increasing concentrations of rapamycin (C, left).

Cell lysates of hippocampal tissue from the aged huAPP / PS1 and control mice were analyzed for an effect of J147 on the APP processing pathway by immunoblotting with antibodies against BACE (D) and APP (E).

Distribution of Fc gamma receptors on trophoblast during human placental development: an immunohistochemical and immunoblotting study.

Immunoblotting assays were carried out by standard procedures using Snail (Cell Signaling Technology, Inc.) and vimentin antibodies (Novus Biologicals, Littleton, CO, USA).

After 48 hours of transfection, cell extracts were prepared and the expression level of FLAG - DDX3, p21waf1 / cip1, and α - actin was detected by immunoblotting with anti-FLAG, anti-p21waf1 / cip1, and anti-α-actin (Santa Cruz Biotechnology, Santa Cruz, CA) antibodies.

Immunoblots were interpreted by criteria of the Centers for Disease Control and the Association of State and Territorial Public Health Laboratory Directors [31].

(E) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC) of parental 1833 and SCP28 cells.

(K) Representative immunoblots of 1833 cells transfected with control shRNA, shRNA against ABL1 (shABL1), ABL2 (shABL2), and shRNA # 2 against both ABL1 and ABL2 (shAA # 2) and ABL1 / ABL2 knockdown cells with overexpression of mouse Abl1 / Abl2 (shAA + mAbl1 / Abl2).

(I) Immunoblotting was performed using the indicated antibodies on whole - cell lysates from cells incubated or not with TRAIL.

Cell lysates were harvested and subjected to immunoblot analysis for MEK1, MEK2, MKK3, MKK6, phosphorylated ERK1 / 2 (p - ERK1 / 2), total ERK1 / 2 (t - ERK1 / 2), phosphorylated p38 (p - p38), total p38 (t - p38) and actin.

(K) Immunoblots were performed on whole - cell lysate.

(A to C) Immunoblots with the indicated antibodies were performed on whole - cell lysates of 1833 and SCP28 cells.

Brain, eyes, and optic nerves were taken from transgenic and wild - type mice of 3 to 12 months of age and processed for immunohistochemistry, qPCR, or western immunoblotting.

(D) Immunoblots were performed on whole - cell lysates (pSTAT5, STAT5, and tubulin) or conditioned medium (MMP1, IL - 6, and TNC).

Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.

GAPDH, glyceraldehyde -3-phosphate dehydrogenase; IB, immunoblotting.

Levels of MEK1, MEK2, MKK3, MKK6 and actin were analyzed by immunoblot analysis.

Immunoblot analysis using mouse monoclonal antibody to human IgM detected IgM to cytomegalovirus (CMV)- specific proteins (150, 42, 38, 32, and 28 kDa) in 74 (38 %) of 197 seropositive serum samples from 197 individuals in three subject groups: 43 surgical patients, 31 patients with solid tumors, and 123 healthy individuals.

We also provide assistance with gel - based protein separation, western immunoblotting, gel / membrane imaging.

(A) Immunoblot illustration of the use of RIPK2 phospho - antibodies in two Hodgkin's lymphoma cells that have constitutive active RIPK2.

IB, immunoblotting.

(D) Protein carbonyls were detected at different concentrations (2.5, 5, 10 pM) as (DNP) hydrazone adducts via immunoblots with a DNP antibody.

A band consistent with the entire ectodomain was observed by immunoblotting for the majority of the proteins and their expression levels were quantified.

2 days after release of UO or at the corresponding time point in the non-UO sham - operated mice, the left kidneys were harvested and subjected to immunoblot analyses of HIF - 2α and co-detection of TBP as a loading control

We assayed inflammasome activation by immunoblotting and ELISA to detect IL - 1β processing and LDH release to determine pyroptosis.

Samples were prepared for cell cycle analysis and immunoblotting.

Lysates were harvested 3 days after transfection and subjected to immunoblotting.