Sentences with phrase «id1 promoter activity»

When fused to an activation domain, ZFHD1 regulated promoter activity in vivo in a sequence - specific manner.

Follicle - stimulating hormone receptor gene promoter activity.

A transcriptional regulatory element critical for CHRNB4 promoter activity in vivo.

DDX3 enhances p21waf1 / cip1 gene expression through up - regulation of promoter activity of p21waf1 / cip1.

DDX3 enhances the expression of p21waf1 / cip1 gene and up - regulates the promoter activity of p21waf1 / cip1 through an ATPase - dependent but helicase - independent mechanism.

To examine whether DDX3 transactivates the p21waf1 / cip1 promoter activity through Sp1 site - interacting proteins, the Sp1 or Sp3 expression plasmid together with the DDX3 expression construct and the p21waf1 / cip1 promoter — driven luciferase reporter were introduced into HuH - 7 cells.

Interestingly, our data revealed that at a higher dosage (2 μg / well), the wild - type DDX3 and mutant DDX3 / AAA up - regulated the activity of p21waf1 / cip1 promoter ∼ 12-fold whereas the DDX3 / DQAD mutant only activated the p21waf1 / cip1 promoter activity ∼ 5-fold (Supplementary Fig.

Furthermore, DDX3 interacted and cooperated with Sp1 to up - regulate the promoter activity of p21waf1 / cip1.

To address the mechanism of growth inhibition by DDX3, we examined whether the promoter activity and the expression level of the cell cycle regulator p21waf1 / cip1 are regulated by DDX3.

As shown in Fig. 4A, transient expression of DDX3 alone resulted in a 4-fold activation of p21waf1 / cip1 promoter activity whereas transiently expressed Sp1 or Sp3 of increasing amount led to a 1.6 - to 1.8-fold activation of the p21waf1 / cip1 promoter.

This observation is consistent with our in vitro studies (see Figs. 2 and 3), which indicate that DDX3 up - regulates p21waf1 / cip1 promoter activity in a p53 - independent manner.

A, DDX3 up - regulates the p21waf1 / cip1 promoter activity.

Promoter activities were expressed as relative luciferase units calculated as the ratio of firefly luciferase to renilla luciferase.

A conserved core element is functionally important for maize mitochondrial promoter activity in vitro

COX - 2 promoter activity was increased to 158 % with the presence of the STAT6 binding site, whereas it remained unchanged with the absence of the STAT6 binding site (Figure 6a), suggesting the STAT6 binding site in the COX - 2 promoter is essential for the function of unphosphorylated STAT6.

This design permits excision of all but 238 bp of inert proviral DNA, lacking both promoter activity and polyadenylation signals, following cleavage by Cre.

A common FADS2 promoter polymorphism increases promoter activity and facilitates binding of transcription factor ELK1

Not exact matches

«That's because the focus of NPS is on creating promoters, but stops short of engaging them to actually promote the business through activities like referrals, references, blogging or tweeting, speaking at industry events, or any of the myriad ways that passionate customers can help build businesses these days.»

«BRAC promoters focus on immunization and family planning in addition to standard Integrated Community Case Management (ICCM) activities (which largely focus on treating malaria and diarrhea, while identifying and making referrals for pneumonia).»

Each promoter needs to consider whether their activities amount to regulated activities under the relevant law.

I / WE HEREBY RELEASE, WAIVE, DISCHARGE AND COVENANT NOT TO SUE the CHICAGO SPORT & SOCIAL CLUB, INC. («CSSC») and its affiliates (CSSC and its affiliates are referred to collectively as the «CLUB»), the sufficiency of which consideration is expressly acknowledged, and intending to be legally bound, do hereby, for myself, my heirs, executors, administrators, insurers, assigns, attorneys, representatives, agents, beneficiaries, legatees, representatives, successors, assigns and any other persons who may make claims on my behalf (collectively the «RELEASORS») OR ALL SPORTS SERIES / PARK DISTRICT OF HIGHLAND PARK / WINDY CITY FIELD HOUSE / CHICAGO PARK DISTRICT / FITNESS FORMULA CLUBS (FFC — UNION STATION) / URBANA PARK DISTRICT / MADISON PARKS ORGANIZATION / ABUNDANT LIFE CHRISTIAN SCHOOL / LANSING PARKS / CITY OF BLOOMINGTON / UNIVERSITY OF MICHIGAN / CHICAGO PUBLIC SCHOOLS — LAKE VIEW HIGH SCHOOL / CAMP OJIBWA / AUSTIN PARKS AND RECREATION facilities used by the participant, including its owners, managers, promoters, lessees of premises used to conduct the event or program, premises and event inspectors, underwriters, consultants and others who give recommendations, directions, or instructions to engage in risk evaluation or loss control activities regarding the CHICAGO SPORT & SOCIAL CLUB, INC. («CSSC») and its affiliates (CSSC and its affiliates are referred to collectively as the «CLUB»), the sufficiency of which consideration is expressly acknowledged, and intending to be legally bound, do hereby, for myself, my heirs, executors, administrators, insurers, assigns, attorneys, representatives, agents, beneficiaries, legatees, representatives, successors, assigns and any other persons who may make claims on my behalf (collectively the «RELEASORS») OR ALL SPORTS SERIES / PARK DISTRICT OF HIGHLAND PARK / WINDY CITY FIELD HOUSE / CHICAGO PARK DISTRICT / FITNESS FORMULA CLUBS (FFC — UNION STATION) / URBANA PARK DISTRICT / MADISON PARKS ORGANIZATION / ABUNDANT LIFE CHRISTIAN SCHOOL / LANSING PARKS / CITY OF BLOOMINGTON / UNIVERSITY OF MICHIGAN / CHICAGO PUBLIC SCHOOLS — LAKE VIEW HIGH SCHOOL / CAMP OJIBWA / AUSTIN PARKS AND RECREATION facilities or events held at such facility and each of them, their directors, officers, agents, employees, all for the purposes herein referred to as «Releasee»... FROM ALL LIABILITY TO THE UNDERSIGNED, my / our personal representatives, assigns, executors, heirs and next to kin FOR ANY AND ALL CLAIMS, DEMANDS, LOSSES OR DAMAGES AND ANY CLAIMS OR DEMANDS THEREFORE ON ACCOUNT OF ANY INJURY, INCLUDING BUT NOT LIMITED TO THE DEATH OF THE PARTICIPANT OR DAMAGE TO PROPERTY, ARISING OUT OF OR RELATING TO THE EVENT (S) CAUSED OR ALLEGED TO BE CAUSED IN WHOLE OR IN PART BY THE NEGLIGENCE OF THE RELEASEE OR OTHERWISE.

Trained community - based growth promoters to promote EBF; pregnant women's support groups and multisectoral activities promoted nutrition behaviors

«Why should promoters be allowed to get rich from this violent and disturbing activity and then kick the damaged ex-fighters to the curb for the taxpayers to take responsibility for in future years.»

BIOFAB uses a technique devised by one of Endy's former students, Jason Kelly, in which researchers determine the relative activity of their promoter based on a widely used reference promoter.

The second insight involved changes in the evolution of gene regulation: As social complexity increased, so did the speed of changes to parts of the genome involved in regulating gene activity, located in the promoters of the genes, the researchers report.

«Using this system, we can visualize the expression of a fluorescent protein and monitor the transcriptional activity from the E-cadherin promoter region as fluorescent intensity, allowing the screening of EMT - suppressive miRNAs.»

Monsanto holds the rights to several patents, including a key piece of DNA, called the 35S promoter, used to promote activity of the beta - carotene genes.

Just as engineers rearrange capacitors and resistors to create different electric circuits, researchers can arrange gene promoters and repressors — stretches of DNA that control gene activity — to create gene circuits with different properties.

The protein, HSF - 1 (heat shock factor - 1), does this by binding to promoters upstream of the 350 - plus chaperone genes, upping the genes» activity and launching the army of chaperones, which originally were called «heat shock proteins.»

Using bioinformatics tools to identify and map out specific components and regulatory interconnections, the study team found highly dynamic activities during CD8 + T cell responses: a distinct repertoire of super enhancers — groups of enhancers that interact with promoters to drive gene transcription, new groups of enhancers that jump into activity only in the memory cell stage, and extensive re-wiring of regulatory circuits from one cell stage to another.

These sequences in our DNA are often situated far away from the actual genes they regulate, in comparison to so called promoter regions, which traditionally have been considered to control most of the gene activity.

To determine whether FLRG can also inhibit myostatin activity in vivo, I generated a construct in which the FLRG coding sequence was placed downstream of a myosin light chain promoter / enhancer.

Looking across all reference epigenomes, about 2.3 million regions (12.6 % of the genome) showed evidence of promoter or enhancer activity in at least one cell or tissue type.

The mechanism by which MNLPs modify gene activity is not yet known; a leading hypothesis is that the short sequences change a promoter's ability to bind proteins that regulate transcription.

Temporally - and spatially - regulated transcriptional activity of the nicotinic acetylcholine receptor [beta] 4 subunit gene promoter.

The in vitro activity and specificity of human endothelial cell - specific promoters in porcine cells.

The results suggested that the levels of activation induced by DDX3 of the luciferase activity of serial reporter constructs from (− 1,002 / +8) p21 - Luc to (− 123 / +8) p21 - Luc were comparable with the level of activation elicited by the full - length promoter - driven reporter p21 - Luc (5 - to 10-fold; Fig. 3B and Supplementary Fig.

The ATPase activity, but not the helicase activity, of DDX3 contributes to DDX3 - mediated transcriptional modulation on the p21waf1 / cip1 promoter.

Because DDX3 harbors growth - suppressive ability and transcriptional modulation activity of the p21waf1 / cip1 promoter, in this work we further examined the deregulated expression of DDX3 in tumors by a cancer profiling array and immunohistochemical staining analysis.

PULLMAN — Researchers at Washington State University have identified a new class of DNA sequence variation in gene promoter regions that could help control the activity of genes.The novel variations, dubbed «multiple nucleotide length polymorphisms,» or MNLPs, altered transcription of the genes they were associated with as much as 11-fold.

As shown in Fig. 2A, exogenous expression of DDX3 in various cell lines led to a 2 - to 4-fold up - regulation of p21waf1 / cip1 promoter — driven luciferase activity.

There is, however, another mechanism by which ARE - regulated genes are controlled and that is through Bach1, a transcriptional repressor that binds to ARE promoter elements resulting in suppression of Nrf2 activity.

Solberg N, Krauss S (2013) Luciferase assay to study the activity of a cloned promoter DNA fragment Methods Mol Biol, 977, 65 - 78 PubMed 23436354

A Strategy for Enhancing the Transcriptional Activity of Weak Cell Type - Specific Promoters.

Single - cell experiments show that gene expression is random and «bursty,» Lin said, a feature that can emerge from slow switching between promoter states with different activities.

Similarly, another group had reported excessive hair growth in mice with genetically augmented TERT activity under an unphysiologic promoter, (5) suggesting a possible role of TERT in follicular (and possibly stem) cell growth.

To gain insight into how mammalian gene expression is controlled by rapidly evolving regulatory elements, we jointly analysed promoter and enhancer activity with downstream transcription levels in liver samples from 15 species.

Selection for expression of the gene requires transcription from a cellular promoter, and consequently a mutation in a cellular gene, and the activity of the tagged gene can be followed by staining for beta galactosidase activity.

Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter.

The resource provides services including: processing of the samples (cells or tissues) to make high quality chromatin for ChIP, ChIP with a set of validated antibodies for histone marks (Promoter marks: H3K4me3 and H3K27me3, Enhancer marks: H3K4me1 and H3K27ac, Gene body marks for transcription activity: H3K36me3 and H3K79me2, and Heterochromatin mark: H3K9me3), ChIP with investigator preferred custom antibodies, and library preparation from ChIP DNA and multi-plexing for high - throughput DNA sequencing.