Not exact matches
Isotype control antibody (black line) was rabbit
IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.
Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 -
IgG, trastuzumab, or
isotype control antibody.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the
IgG1
Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse
IgG1,
IgG2a,
IgG2b,
IgG3, IgM and heavy and light chains (H&L) of
IgG.
To assess the ability of F3 -
IgG to inhibit the proliferation of HER2 - overexpressing breast carcinoma cells, we measured the proliferation of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence of trastuzumab, F3 -
IgG, or an
isotype - matched
control antibody.