Sentences with phrase «igg isotypes»

aureus IgG isotypes influences surface protein A mediated immune subversion.

Not exact matches

By contrast, the anti-GalC antibodies in the GBS patients were of the isotype IgG.

In addition, rabbits only have one isotype of IgG molecules with more disulphide bonds.

The secondary antibody must be specific to the isotype class of the primary antibody (e.g., IgG or IgM)

Isotype control antibody (black line) was rabbit IgG (monoclonal)(1μg / 1x106 cells) used under the same conditions.

An isotype control IgG was run in parallel and showed no positive staining (not shown here).

They were then washed in FACS buffer I and probed in FACS buffer I containing a 1:500 dilution of r - phycoerythrin - conjugated goat anti-mouse IgG (Caltag Laboratories, Carlsbad, CA) or an appropriate isotype control for 15 min in the dark at 4 °C.

Inhibition of cell proliferation (B) of MCF7, MCF7 / HER2, or BT474 cells treated for 6 days with F3 - IgG, trastuzumab, or isotype control antibody.

An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1 Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3, IgM and heavy and light chains (H&L) of IgG.

Breast carcinoma cells (5 × 103 cells / well) were seeded into a 96 - well plate and treated with trastuzumab, isotype - matched control IgG, or F3 - IgG at concentrations ranging from 0.01 to 10 µg / mL.

To assess the ability of F3 - IgG to inhibit the proliferation of HER2 - overexpressing breast carcinoma cells, we measured the proliferation of BT474, MCF7 / HER2 and MCF7 breast cancer cell lines in the presence of trastuzumab, F3 - IgG, or an isotype - matched control antibody.