However, these were all of the antibody
isotype M (immunoglobulin M,
IgM), the earliest antibody type elicited during an acute immune response.
An isotyping ELISA was performed by coating a 96 - well plate with 1.25 ug / mL of the IgG1
Isotype Control Antibody and detecting with Alexa Fluor conjugates specific to mouse IgG1, IgG2a, IgG2b, IgG3,
IgM and heavy and light chains (H&L) of IgG.