The Illumina library preparation and sequencing was done by the Cornell Biotechnology Resource Center.
Not exact matches
Using subjects selected by Dr. Susan Levine (Manhattan, NY), Dr. Rita Shaknovich's lab (Weill Cornell Medical School) has purified B, T, and NK cells for
preparation of mRNAs, which will be characterized through
Illumina sequencing of cDNA
libraries at the Cornell Sequencing Facility and analyzed by Dr. Fabien Campagne's group (Weill Cornell Medical School).
The
preparation of barcoded
Illumina sequencing
libraries was performed according to Meyer and Kircher [62] and hybridized to the Agilent arrays following the protocol described by Hodges et al. [60].
Running as facility of this size requires a massive amount of support and we work closely with the
library preparation team that supplies large numbers of DNA templates in a from ready to be sequenced, the Institute's IT team that maintains the extensive amount of compute and storage infrastructure necessary, sequencing informatics which develops software tools to process, analyse, store and track all the data, projects and samples for the
Illumina pipeline and the development team which invents novel and improved protocols to take better advantage of this new technology.