Sentences with phrase «immunoblot analysis of»
Immunoblot analysis of recombinant antigens and B. burgdorferi lysates with representative sera from mice inoculated by i.p. / s.c. routes with B31 - A3 WT, ospC7 mutant, and ospC7 / ospC +4 complemented B. burgdorferi clones.
http://www.pnas.org/
Similar protocols were used for immunoblot analysis of cell protein extracts from MC3T3 - E1 (mouse preosteoblasts), U-2 OS (human osteosarcoma), and C2C12 (mouse myoblasts with osteogenic potential).
2 days after release of UO or at the corresponding time point in the non-UO sham - operated mice, the left kidneys were harvested and subjected to immunoblot analyses of HIF - 2α and co-detection of TBP as a loading control
Not exact matches
Protein immunoblot and histochemical analysis with antiserum to type I NO synthase suggest that the formation of NO in pancreatic B cells is catalyzed by an NADPH -(reduced form of nicotinamide adenine dinucleotide phosphate), Ca2 + / calmodulin - dependent type I NO synthase of about 150 kilodaltons.
Levels of MEK1, MEK2, MKK3, MKK6 and actin were analyzed by immunoblot analysis.
Immunoblot analysis using mouse monoclonal antibody to human IgM detected IgM to cytomegalovirus (CMV)- specific proteins (150, 42, 38, 32, and 28 kDa) in 74 (38 %) of 197 seropositive serum samples from 197 individuals in three subject groups: 43 surgical patients, 31 patients with solid tumors, and 123 healthy individuals.