Sentences with phrase «immunoprecipitation with»

(B) Validation of fgf12 and PDE3A as bona fide Oct4 targets by chromatin immunoprecipitation with either serum (s, control) or monoclonal (1) or polyclonal (2) antibodies against Oct4.

Immunoprecipitation with antibodies against dimethyl - K4 H3 was limited to the edges of the contig flanking the dimethyl - K9 H3 region [6].

To measure bits of amyloid coursing through the bloodstream, Yanagisawa and colleagues used a technique called immunoprecipitation with mass spectrometry, which deploysantibodies to bind and identify proteins.

Not exact matches

One current method commonly used today is called chromatin immunoprecipitation, combined with next - generation gene sequencing (ChIP - seq).

At ABclonal, we have nearly 100 existing chromatin immunoprecipitation (ChIP)- validated antibodies with a higher specificity than most commercial antibodies, which are used for a wide range of epigenetics studies, from those investigating developmental processes to those researching various diseases.

To this end, using knockout cells in combination with siRNA and immunoprecipitation approaches, our results revealed that Pen - 2 together with presenilin are sufficient... to form a functionally active enzyme to process Notch.

(e, g) Western blot (WB) analysis of tyrosine phosphorylation (pTyr) and total Tie2 after Tie2 immunoprecipitation (IP) in BP (e) and HUVEC (g) upon stimulation with recombinant human (rh) Ang1 compared to unstimulated (us) cells.

After extensive washing with immunoprecipitation lysis buffer, the immunoprecipitated proteins were analyzed by immunoblotting using specific antibodies against DDX3 and FLAG epitope (ab1257, Abcam, Cambridge, United Kingdom).

BRCTx binds to the C terminus of hRAD18 in yeast two - hybrid and immunoprecipitation assays and colocalizes with this protein in the nucleus.

For the coimmunoprecipitation experiments, HuH - 7 cells transfected with pcDNA - SRα / FLAG - Sp 1 were harvested and cell lysates were prepared using immunoprecipitation lysis buffer [20 mmol / L Tris - Cl (pH 7.5), 150 mmol / L NaCl, 10 % glycerol, and 1 % Triton X-100].

By using immunoprecipitation and mass spectrometry, we identified autoantibodies anti-Adenylate kinase 5 (AK5 - Abs) as specific marker in 10 patients with LE.

(D) Cells transfected with either Keap1 or control siRNA were subjected to chromatin immunoprecipitation after treatment with either 10 µM HPP - 1014, 10 µM CoPP, or 1 µM HPP - 4382 for 6 hours.

STAT6 co-immunoprecipitated with the transcriptional co-activator, p300, and chromatin immunoprecipitation assays demonstrated that these proteins bind a consensus STAT6 binding site located within the COX - 2 promoter.

Furthermore, by chromatin immunoprecipitation and whole - genome scanning approaches, we show that the chromosome ends of senescent cells directly contribute to the DNA damage response, and that uncapped telomeres directly associate with many, but not all, DNA damage response proteins.

We present here the interactome of Nbeal2 with additional validation by reverse immunoprecipitation of Dock7, Sec16a and Vac14 as interactors of Nbeal2.

(D) Treatment with 1 mM 8 - Br - cAMP for four hours enhances Oct4 binding to genomic loci as determined by chromatin immunoprecipitation, followed by real - time PCR analysis at Nanog and fgf12 sites.

Genome - Wide Methylated DNA Immunoprecipitation Analysis of Patients with Polycystic Ovary Syndrome Hao - ran Shen, 1 Li - hua Qiu, 1 Zhi - qing Zhang, 2 Yuan - yuan Qin, 1 Cong Cao, 2, * andWen Di1, *