Sentences with phrase «increases transduction»

Not exact matches

In addition to the increased collaboration, the intellectual critical mass existing within the IMCB also helped the learning process through stimulating discussions with seasoned experts in exciting topics such as signal transduction and cell cycle processes.

Similarly, Bernaudin et al. [43] found increased expression of 18 genes in the neonatal rat brain following hypoxia (8 % O2 for 3 h) including several known hypoxia inducible genes such as MAP kinase phosphatase - 1 (MKP - 1), several HIF - 1 target genes including VEGF and GLUT - 1, genes implicated in apoptosis, signal transduction molecules, and transcription factors.

The use of viral vectors in research is beneficial for a number of reasons, including but not limited to: helping to get difficult - to - deliver DNA into mammalian cells, increasing the efficiency of gene transduction, allowing for control over which cells are infected through viral pseudotyping, and ease of vector cloning and modification.

Deletion of DGKζ from T cells results in prolonged TCR signal transduction downstream of DAG, resulting in enhanced activation of Ras, increased effector T cell proliferation, and amplified cytokine production, which leads to increased antitumor activity against s.c. implanted EL4 tumors or murine mesothelioma (12, 16, 17).

Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to increase the efficiency of lentiviral transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.

Initial evaluation of this method showed 5 — 10 × increase in transduction efficiency.

The lentivirus - encoded shRNAs decreased the abundance of ABL1 and ABL2 by 80 % at day 2 after viral transduction, but ABL1 abundance was slightly increased by day 21 after viral transduction (fig.

Increased expression of transcription factor ATF2 — which is required for the amino acid depletion responses of genes such as ATF3, CHOP, SARS and 4EBP - 1 — is mediated by a signal transduction pathway that appears to involve an amino acid sensing GPR [37].

Both transient transfection with Lipofectamine and transduction with adenovirus resulted in a subpopulation of cells that experessed human IgG, as shown by the increased fluorescence intensity.