The use of viral vectors in research is beneficial for a number of reasons, including but not limited to: helping to get difficult - to - deliver DNA into mammalian cells,
increasing the
efficiency of gene
transduction, allowing for control over which cells are infected through viral pseudotyping, and ease of vector cloning and modification.
Whether a protocol uses cell culture methods to expand edited cells for downstream assays, to
increase the
efficiency of lentiviral
transduction or to let cells «rest» after electroporation, optimized culture conditions are key to maintaining cell viability and biological function.